Calbindin D28K interacts with Ran-binding protein M: identification of interacting domains by NMR spectroscopy.

Journal Article (Journal Article)

Calbindin D(28K) is an EF-hand containing protein that plays a vital role in neurological function. We now show that calcium-loaded calbindin D(28K) interacts with Ran-binding protein M, a protein known to play a role in microtubule function. Using NMR methods, we show that a peptide, LASIKNR, derived from Ran-binding protein M, interacts with several regions of the calcium-loaded protein including the amino terminus and two other regions that exhibit conformational exchange on the NMR timescale. We suggest that the interaction between calbindin D(28K) and Ran-binding protein M may be important in calbindin D(28K) function.

Full Text

Duke Authors

Cited Authors

  • Lutz, W; Frank, EM; Craig, TA; Thompson, R; Venters, RA; Kojetin, D; Cavanagh, J; Kumar, R

Published Date

  • April 18, 2003

Published In

Volume / Issue

  • 303 / 4

Start / End Page

  • 1186 - 1192

PubMed ID

  • 12684061

International Standard Serial Number (ISSN)

  • 0006-291X

Digital Object Identifier (DOI)

  • 10.1016/s0006-291x(03)00499-6

Language

  • eng

Conference Location

  • United States