Pollen-derived RNAs Are Found in the Human Circulation.

Journal Article (Journal Article)

The presence of nonhuman RNAs in man has been questioned and it is unclear if food-derived miRNAs cross into the circulation. In a large population study, we found nonhuman miRNAs in plasma by RNA sequencing and validated a small number of pine-pollen miRNAs by RT-qPCR in 2,776 people. The presence of these pine-pollen miRNAs associated with hay fever and not with overt cardiovascular or pulmonary disease. Using in vivo and in vitro models, we found that transmission of pollen-miRNAs into the circulation occurs via pulmonary transfer and this transfer was mediated by platelet-pulmonary vascular cell interactions and platelet pollen-DNA uptake. These data demonstrate that pollen-derived plant miRNAs can be horizontally transferred into the circulation via the pulmonary system in humans. Although these data suggest mechanistic plausibility for pulmonary-mediated plant-derived miRNA transfer into the human circulation, our large observational cohort data do not implicate major disease or risk factor association.

Full Text

Duke Authors

Cited Authors

  • Koupenova, M; Mick, E; Corkrey, HA; Singh, A; Tanriverdi, SE; Vitseva, O; Levy, D; Keeler, AM; Ezzaty Mirhashemi, M; ElMallah, MK; Gerstein, M; Rozowsky, J; Tanriverdi, K; Freedman, JE

Published Date

  • September 27, 2019

Published In

Volume / Issue

  • 19 /

Start / End Page

  • 916 - 926

PubMed ID

  • 31518900

Pubmed Central ID

  • PMC6742912

Electronic International Standard Serial Number (EISSN)

  • 2589-0042

Digital Object Identifier (DOI)

  • 10.1016/j.isci.2019.08.035

Language

  • eng

Conference Location

  • United States