Fine mapping of the Pc locus of Sorghum bicolor, a gene controlling the reaction to a fungal pathogen and its host-selective toxin.

Published

Journal Article

Milo disease in sorghum is caused by isolates of the soil-borne fungus Periconia circinata that produce PC-toxin. Susceptibility to milo disease is conditioned by a single, semi-dominant gene, termed Pc. The susceptible allele (Pc) converts to a resistant form (pc) spontaneously at a gametic frequency of 10(-3) to 10(-4). A high-density genetic map was constructed around the Pc locus using DNA markers, allowing the Pc gene to be delimited to a 0.9 cM region on the short arm of sorghum chromosome 9. Physically, the Pc-region was covered by a single BAC clone. Sequence analysis of this BAC revealed twelve gene candidates. Several of the predicted genes in the region are homologous to disease resistance loci, including one NBS-LRR resistance gene analogue that is present in multiple tandem copies. Analysis of pc isolines derived from Pc/Pc sorghum suggests that one or more members of this NBS-LRR gene family are the Pc genes that condition susceptibility.

Full Text

Duke Authors

Cited Authors

  • Nagy, ED; Lee, T-C; Ramakrishna, W; Xu, Z; Klein, PE; SanMiguel, P; Cheng, C-P; Li, J; Devos, KM; Schertz, K; Dunkle, L; Bennetzen, JL

Published Date

  • April 2007

Published In

Volume / Issue

  • 114 / 6

Start / End Page

  • 961 - 970

PubMed ID

  • 17356869

Pubmed Central ID

  • 17356869

International Standard Serial Number (ISSN)

  • 0040-5752

Digital Object Identifier (DOI)

  • 10.1007/s00122-006-0481-1

Language

  • eng

Conference Location

  • Germany