Angiopellosis as an Alternative Mechanism of Cell Extravasation.

Journal Article (Journal Article)

Stem cells possess the ability to home in and travel to damaged tissue when injected intravenously. For the cells to exert their therapeutic effect, they must cross the blood vessel wall and enter the surrounding tissues. The mechanism of extravasation injected stem cells employ for exit has yet to be characterized. Using intravital microscopy and a transgenic zebrafish line Tg(fli1a:egpf) with GFP-expressing vasculature, we documented the detailed extravasation processes in vivo for injected stem cells in comparison to white blood cells (WBCs). While WBCs left the blood vessels by the standard diapedesis process, injected cardiac and mesenchymal stem cells underwent a distinct method of extravasation that was markedly different from diapedesis. Here, the vascular wall undergoes an extensive remodeling to allow the cell to exit the lumen, while the injected cell remains distinctively passive in activity. We termed this process Angio-pello-sis, which represents an alternative mechanism of cell extravasation to the prevailing theory of diapedesis. Stem Cells 2017;35:170-180 Video Highlight: https://youtu.be/i5EI-ZvhBps.

Full Text

Duke Authors

Cited Authors

  • Allen, TA; Gracieux, D; Talib, M; Tokarz, DA; Hensley, MT; Cores, J; Vandergriff, A; Tang, J; de Andrade, JBM; Dinh, P-U; Yoder, JA; Cheng, K

Published Date

  • January 2017

Published In

Volume / Issue

  • 35 / 1

Start / End Page

  • 170 - 180

PubMed ID

  • 27350343

Pubmed Central ID

  • PMC5376103

Electronic International Standard Serial Number (EISSN)

  • 1549-4918

International Standard Serial Number (ISSN)

  • 1066-5099

Digital Object Identifier (DOI)

  • 10.1002/stem.2451

Language

  • eng