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Gene Expression of Haloferax volcanii on Intermediate and Abundant Sources of Fixed Nitrogen.

Publication ,  Journal Article
Hwang, S; Chavarria, NE; Hackley, RK; Schmid, AK; Maupin-Furlow, JA
Published in: International journal of molecular sciences
September 2019

Haloferax volcanii, a well-developed model archaeon for genomic, transcriptomic, and proteomic analyses, can grow on a defined medium of abundant and intermediate levels of fixed nitrogen. Here we report a global profiling of gene expression of H. volcanii grown on ammonium as an abundant source of fixed nitrogen compared to l-alanine, the latter of which exemplifies an intermediate source of nitrogen that can be obtained from dead cells in natural habitats. By comparing the two growth conditions, 30 genes were found to be differentially expressed, including 16 genes associated with amino acid metabolism and transport. The gene expression profiles contributed to mapping ammonium and l-alanine usage with respect to transporters and metabolic pathways. In addition, conserved DNA motifs were identified in the putative promoter regions and transcription factors were found to be in synteny with the differentially expressed genes, leading us to propose regulons of transcriptionally co-regulated operons. This study provides insight to how H. volcanii responds to and utilizes intermediate vs. abundant sources of fixed nitrogen for growth, with implications for conserved functions in related halophilic archaea.

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Published In

International journal of molecular sciences

DOI

EISSN

1422-0067

ISSN

1422-0067

Publication Date

September 2019

Volume

20

Issue

19

Start / End Page

E4784

Related Subject Headings

  • Transcriptome
  • Nitrogen Fixation
  • Nitrogen
  • Metabolic Networks and Pathways
  • Haloferax volcanii
  • Genome-Wide Association Study
  • Gene Expression Regulation, Archaeal
  • Gene Expression Profiling
  • Computational Biology
  • Chemical Physics
 

Citation

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Hwang, S., Chavarria, N. E., Hackley, R. K., Schmid, A. K., & Maupin-Furlow, J. A. (2019). Gene Expression of Haloferax volcanii on Intermediate and Abundant Sources of Fixed Nitrogen. International Journal of Molecular Sciences, 20(19), E4784. https://doi.org/10.3390/ijms20194784
Hwang, Sungmin, Nikita E. Chavarria, Rylee K. Hackley, Amy K. Schmid, and Julie A. Maupin-Furlow. “Gene Expression of Haloferax volcanii on Intermediate and Abundant Sources of Fixed Nitrogen.International Journal of Molecular Sciences 20, no. 19 (September 2019): E4784. https://doi.org/10.3390/ijms20194784.
Hwang S, Chavarria NE, Hackley RK, Schmid AK, Maupin-Furlow JA. Gene Expression of Haloferax volcanii on Intermediate and Abundant Sources of Fixed Nitrogen. International journal of molecular sciences. 2019 Sep;20(19):E4784.
Hwang, Sungmin, et al. “Gene Expression of Haloferax volcanii on Intermediate and Abundant Sources of Fixed Nitrogen.International Journal of Molecular Sciences, vol. 20, no. 19, Sept. 2019, p. E4784. Epmc, doi:10.3390/ijms20194784.
Hwang S, Chavarria NE, Hackley RK, Schmid AK, Maupin-Furlow JA. Gene Expression of Haloferax volcanii on Intermediate and Abundant Sources of Fixed Nitrogen. International journal of molecular sciences. 2019 Sep;20(19):E4784.

Published In

International journal of molecular sciences

DOI

EISSN

1422-0067

ISSN

1422-0067

Publication Date

September 2019

Volume

20

Issue

19

Start / End Page

E4784

Related Subject Headings

  • Transcriptome
  • Nitrogen Fixation
  • Nitrogen
  • Metabolic Networks and Pathways
  • Haloferax volcanii
  • Genome-Wide Association Study
  • Gene Expression Regulation, Archaeal
  • Gene Expression Profiling
  • Computational Biology
  • Chemical Physics