Embryonic Fundulus heteroclitus responses to sediment extracts from differentially contaminated sites in the Elizabeth River, VA.

Journal Article (Journal Article)

Sites along the Elizabeth River are contaminated with polycyclic aromatic hydrocarbons (PAHs) from historical creosote production and other industrial processes. Previous studies have demonstrated that Atlantic killifish collected from sites throughout the Elizabeth River display resistance to the teratogenic effects of PAH-exposure in a manner commensurate with sediment PAH concentrations. The current study characterized various chemical pollutants in sediment and investigated the effects of aqueous sediment extracts from sites along the Elizabeth River to the cardiac development of Atlantic killifish embryos from fish collected from an uncontaminated reference site. Embryonic cardiac deformities were more prevalent after exposure to extracts from sites with high PAH loads. However, activation of cytochrome P4501A, a gene up-regulated by PAH-induction of the aryl hydrocarbon receptor and measured using an in ovo EROD assay, did not consistently increase with PAH concentrations. This work further characterizes sediments in the Elizabeth River, as well as provides insight into the evolutionary pressures at each ER site.

Full Text

Duke Authors

Cited Authors

  • Volkoff, SJ; Osterberg, JS; Jayasundara, N; Cooper, E; Hsu-Kim, H; Rogers, L; Gehrke, GE; Jayaraman, S; Di Giulio, RT

Published Date

  • November 2019

Published In

Volume / Issue

  • 28 / 9

Start / End Page

  • 1126 - 1135

PubMed ID

  • 31620948

Pubmed Central ID

  • PMC7768634

Electronic International Standard Serial Number (EISSN)

  • 1573-3017

International Standard Serial Number (ISSN)

  • 0963-9292

Digital Object Identifier (DOI)

  • 10.1007/s10646-019-02116-z


  • eng