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Insertional mutagenesis in the zoonotic pathogen Chlamydia caviae.

Publication ,  Journal Article
Filcek, K; Vielfort, K; Muraleedharan, S; Henriksson, J; Valdivia, RH; Bavoil, PM; Sixt, BS
Published in: PLoS One
2019

The ability to introduce targeted genetic modifications in microbial genomes has revolutionized our ability to study the role and mode of action of individual bacterial virulence factors. Although the fastidious lifestyle of obligate intracellular bacterial pathogens poses a technical challenge to such manipulations, the last decade has produced significant advances in our ability to conduct molecular genetic analysis in Chlamydia trachomatis, a major bacterial agent of infertility and blindness. Similar approaches have not been established for the closely related veterinary Chlamydia spp., which cause significant economic damage, as well as rare but potentially life-threatening infections in humans. Here we demonstrate the feasibility of conducting site-specific mutagenesis for disrupting virulence genes in C. caviae, an agent of guinea pig inclusion conjunctivitis that was recently identified as a zoonotic agent in cases of severe community-acquired pneumonia. Using this approach, we generated C. caviae mutants deficient for the secreted effector proteins IncA and SinC. We demonstrate that C. caviae IncA plays a role in mediating fusion of the bacteria-containing vacuoles inhabited by C. caviae. Moreover, using a chicken embryo infection model, we provide first evidence for a role of SinC in C. caviae virulence in vivo.

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Published In

PLoS One

DOI

EISSN

1932-6203

Publication Date

2019

Volume

14

Issue

11

Start / End Page

e0224324

Location

United States

Related Subject Headings

  • Zoonoses
  • Vero Cells
  • Mutation
  • Mutagenesis, Insertional
  • Introns
  • Humans
  • Hela Cells
  • HeLa Cells
  • General Science & Technology
  • Chlorocebus aethiops
 

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Filcek, K., Vielfort, K., Muraleedharan, S., Henriksson, J., Valdivia, R. H., Bavoil, P. M., & Sixt, B. S. (2019). Insertional mutagenesis in the zoonotic pathogen Chlamydia caviae. PLoS One, 14(11), e0224324. https://doi.org/10.1371/journal.pone.0224324
Filcek, Kimberly, Katarina Vielfort, Samada Muraleedharan, Johan Henriksson, Raphael H. Valdivia, Patrik M. Bavoil, and Barbara S. Sixt. “Insertional mutagenesis in the zoonotic pathogen Chlamydia caviae.PLoS One 14, no. 11 (2019): e0224324. https://doi.org/10.1371/journal.pone.0224324.
Filcek K, Vielfort K, Muraleedharan S, Henriksson J, Valdivia RH, Bavoil PM, et al. Insertional mutagenesis in the zoonotic pathogen Chlamydia caviae. PLoS One. 2019;14(11):e0224324.
Filcek, Kimberly, et al. “Insertional mutagenesis in the zoonotic pathogen Chlamydia caviae.PLoS One, vol. 14, no. 11, 2019, p. e0224324. Pubmed, doi:10.1371/journal.pone.0224324.
Filcek K, Vielfort K, Muraleedharan S, Henriksson J, Valdivia RH, Bavoil PM, Sixt BS. Insertional mutagenesis in the zoonotic pathogen Chlamydia caviae. PLoS One. 2019;14(11):e0224324.

Published In

PLoS One

DOI

EISSN

1932-6203

Publication Date

2019

Volume

14

Issue

11

Start / End Page

e0224324

Location

United States

Related Subject Headings

  • Zoonoses
  • Vero Cells
  • Mutation
  • Mutagenesis, Insertional
  • Introns
  • Humans
  • Hela Cells
  • HeLa Cells
  • General Science & Technology
  • Chlorocebus aethiops