Insertional mutagenesis in the zoonotic pathogen Chlamydia caviae.

Published online

Journal Article

The ability to introduce targeted genetic modifications in microbial genomes has revolutionized our ability to study the role and mode of action of individual bacterial virulence factors. Although the fastidious lifestyle of obligate intracellular bacterial pathogens poses a technical challenge to such manipulations, the last decade has produced significant advances in our ability to conduct molecular genetic analysis in Chlamydia trachomatis, a major bacterial agent of infertility and blindness. Similar approaches have not been established for the closely related veterinary Chlamydia spp., which cause significant economic damage, as well as rare but potentially life-threatening infections in humans. Here we demonstrate the feasibility of conducting site-specific mutagenesis for disrupting virulence genes in C. caviae, an agent of guinea pig inclusion conjunctivitis that was recently identified as a zoonotic agent in cases of severe community-acquired pneumonia. Using this approach, we generated C. caviae mutants deficient for the secreted effector proteins IncA and SinC. We demonstrate that C. caviae IncA plays a role in mediating fusion of the bacteria-containing vacuoles inhabited by C. caviae. Moreover, using a chicken embryo infection model, we provide first evidence for a role of SinC in C. caviae virulence in vivo.

Full Text

Duke Authors

Cited Authors

  • Filcek, K; Vielfort, K; Muraleedharan, S; Henriksson, J; Valdivia, RH; Bavoil, PM; Sixt, BS

Published Date

  • 2019

Published In

Volume / Issue

  • 14 / 11

Start / End Page

  • e0224324 -

PubMed ID

  • 31697687

Pubmed Central ID

  • 31697687

Electronic International Standard Serial Number (EISSN)

  • 1932-6203

Digital Object Identifier (DOI)

  • 10.1371/journal.pone.0224324

Language

  • eng

Conference Location

  • United States