In vivo detection of microRNA within plants using plasmonic nanosensors


Conference Paper

© 2019 SPIE. Gene expression monitoring within whole plants is critical for many applications ranging from plant biology to biofuel development. Herein, we report a unique multimodal method for in vivo imaging and biosensing of nucleic acid biotargets, specifically microRNA, within whole plant leaves by integrating three complementary techniques: surface-enhanced Raman scattering (SERS), X-ray fluorescence (XRF), and plasmonics-enhanced two-photon luminescence (TPL). The method described utilizes plasmonic nanostar-based inverse molecular sentinel (iMS) nanoprobes, which not only provide large Raman signal enhancement upon target binding, but also allow for localization and quantification by XRF and plasmonics-enhanced TPL. This report lays the foundation for the use of plasmonic nanoprobes for in vivo functional imaging of nucleic acid biotargets in whole plants.

Full Text

Duke Authors

Cited Authors

  • Crawford, BM; Strobbia, P; Wang, HN; Zentella, R; Boyanov, MI; Pei, ZM; Sun, TP; Kemner, KM; Vo-Dinh, T

Published Date

  • January 1, 2019

Published In

Volume / Issue

  • 11082 /

Electronic International Standard Serial Number (EISSN)

  • 1996-756X

International Standard Serial Number (ISSN)

  • 0277-786X

International Standard Book Number 13 (ISBN-13)

  • 9781510628571

Digital Object Identifier (DOI)

  • 10.1117/12.2529818

Citation Source

  • Scopus