Embryo microinjection of the lecithotrophic sea urchin Heliocidaris erythrogramma .

Journal Article (Journal Article)

Microinjection is a common embryological technique used for many types of experiments, including lineage tracing, manipulating gene expression, or genome editing. Injectable reagents include mRNA overexpression, mis-expression, or dominant-negative experiments to examine a gene of interest, a morpholino antisense oligo to prevent translation of an mRNA or spliceoform of interest and CRISPR-Cas9 reagents. Thus, the technique is broadly useful for basic embryological studies, constructing gene regulatory networks, and directly testing hypotheses about cis-regulatory and coding sequence changes underlying the evolution of development. However, the methods for microinjection in typical planktotrophic marine invertebrates may not work well in the highly modified eggs and embryos of lecithotrophic species. This protocol is optimized for the lecithotrophic sea urchin Heliocidaris erythrogramma .

Full Text

Duke Authors

Cited Authors

  • Edgar, A; Byrne, M; Wray, GA

Published Date

  • January 2019

Published In

Volume / Issue

  • 6 / 3

Start / End Page

  • e119 -

PubMed ID

  • 31772951

Pubmed Central ID

  • PMC6875645

Electronic International Standard Serial Number (EISSN)

  • 2326-9901

International Standard Serial Number (ISSN)

  • 2326-9901

Digital Object Identifier (DOI)

  • 10.14440/jbm.2019.292

Language

  • eng