Burkholderia pseudomallei Detection among Hospitalized Patients, Sarawak.

Published

Journal Article

Burkholderia pseudomallei infections are prevalent in Southeast Asia and northern Australia and often misdiagnosed. Diagnostics are often neither sensitive nor rapid, contributing up to 50% mortality rate. In this 2018 pilot study, we enrolled 100 patients aged 6 months-79 years from Kapit Hospital in Sarawak, Malaysia, with symptoms of B. pseudomallei infection. We used three different methods for the detection of B. pseudomallei: a real-time polymerase chain reaction (PCR) assay, a rapid lateral flow immunoassay, and the standard-of-care bacterial culture-the gold standard. Among the 100 participants, 24 (24%) were positive for B. pseudomallei by one or more of the detection methods. Comparing the two individual diagnostic methods against the gold standard-bacterial culture-of any positive test, there was low sensitivity for each test (25-44%) but high specificity (93-98%). It seems clear that more sensitive diagnostics or a sensitive screening diagnostic followed by specific confirmatory diagnostic is needed for this disease.

Full Text

Duke Authors

Cited Authors

  • Choi, JY; Hii, KC; Bailey, ES; Chuang, JY; Tang, WY; Yuen Wong, EK; Ti, T; Pau, KS; Berita, A; Saihidi, I; Ting, J; Chua, T-T; Toh, T-H; AuCoin, DP; DeShazer, D; Gray, GC

Published Date

  • February 2020

Published In

Volume / Issue

  • 102 / 2

Start / End Page

  • 388 - 391

PubMed ID

  • 31769397

Pubmed Central ID

  • 31769397

Electronic International Standard Serial Number (EISSN)

  • 1476-1645

Digital Object Identifier (DOI)

  • 10.4269/ajtmh.19-0625

Language

  • eng

Conference Location

  • United States