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Membrane sealing copolymer surfactant poloxamer 188 inserts into lipid monolayers at reduced surface pressures

Publication ,  Journal Article
Maskarinec, SA; Hannig, J; Lee, KYC; Lee, RC
Published in: Annals of Biomedical Engineering
December 1, 2000

Experiments were conducted in a Langmuir trough with Wilhelmy plate coupled to a fluorescence microscope. Monolayers of either dipalmitoylphosphatidylcholine (DPPC) and dipalmitoylphosphatidyl-glycerol (DPPG), mimicking the outer cell membrane leaflet, were spread and compressed to 30 mN/m surface pressure, then step-wise relaxed after P188 was injected into the subphase. In pretreatment experiments the monolayer was spread at 0 mN/m and P188 added immediately. then comparessed. At 30°C P188 inserts into both DPPC and DPPG monolayers at surface pressures below 23 mN/m and leaves it once the surface is re-compressed above this value. Similar insertion results for both lipid headgroups suggest the absence of any electrostatic influence. The insertion at surface pressures below that of natural cell membranes (ca. 30 mN/m) indicates that P188 might interact with structurally compromised membranes only, but not with intact cell membranes.

Duke Scholars

Published In

Annals of Biomedical Engineering

ISSN

0090-6964

Publication Date

December 1, 2000

Volume

28

Issue

SUPPL. 1

Related Subject Headings

  • Biomedical Engineering
  • 11 Medical and Health Sciences
  • 09 Engineering
 

Citation

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Maskarinec, S. A., Hannig, J., Lee, K. Y. C., & Lee, R. C. (2000). Membrane sealing copolymer surfactant poloxamer 188 inserts into lipid monolayers at reduced surface pressures. Annals of Biomedical Engineering, 28(SUPPL. 1).
Maskarinec, S. A., J. Hannig, K. Y. C. Lee, and R. C. Lee. “Membrane sealing copolymer surfactant poloxamer 188 inserts into lipid monolayers at reduced surface pressures.” Annals of Biomedical Engineering 28, no. SUPPL. 1 (December 1, 2000).
Maskarinec SA, Hannig J, Lee KYC, Lee RC. Membrane sealing copolymer surfactant poloxamer 188 inserts into lipid monolayers at reduced surface pressures. Annals of Biomedical Engineering. 2000 Dec 1;28(SUPPL. 1).
Maskarinec, S. A., et al. “Membrane sealing copolymer surfactant poloxamer 188 inserts into lipid monolayers at reduced surface pressures.” Annals of Biomedical Engineering, vol. 28, no. SUPPL. 1, Dec. 2000.
Maskarinec SA, Hannig J, Lee KYC, Lee RC. Membrane sealing copolymer surfactant poloxamer 188 inserts into lipid monolayers at reduced surface pressures. Annals of Biomedical Engineering. 2000 Dec 1;28(SUPPL. 1).
Journal cover image

Published In

Annals of Biomedical Engineering

ISSN

0090-6964

Publication Date

December 1, 2000

Volume

28

Issue

SUPPL. 1

Related Subject Headings

  • Biomedical Engineering
  • 11 Medical and Health Sciences
  • 09 Engineering