Mechanically tunable thin films of photosensitive artificial proteins: Preparation and characterization by nanoindentation

Published

Journal Article

Thin films of controlled elastic modulus were made by photo-cross-linking artificial extracellular matrix (aECM) proteins containing the photosensitive amino acid p-azidophenylalanine (pN3Phe). The elastic moduli of the films were calculated from nanoindentation data collected by atomic force microscopy (AFM) using a thin-film Hertz model. The modulus was shown to be tunable in the range 0.3-1.0 MPa either by controlling the irradiation time or by varying the level of pN3Phe in the protein. Tensile measurements on bulk films of the same proteins and finite-element simulation of the indentation process agreed with the thin-film modulus measurements from AFM. Substrates characterized by spatial variation in elastic modulus were created by local control of the irradiation time. © 2008 American Chemical Society.

Full Text

Duke Authors

Cited Authors

  • Nowatzki, PJ; Franck, C; Maskarinec, SA; Ravichandran, G; Tirrell, DA

Published Date

  • March 11, 2008

Published In

Volume / Issue

  • 41 / 5

Start / End Page

  • 1839 - 1845

International Standard Serial Number (ISSN)

  • 0024-9297

Digital Object Identifier (DOI)

  • 10.1021/ma071717a

Citation Source

  • Scopus