Platelet factor 4 and Duffy antigen required for platelet killing of Plasmodium falciparum.

Published

Journal Article

Platelets restrict the growth of intraerythrocytic malaria parasites by binding to parasitized cells and killing the parasite within. Here, we show that the platelet molecule platelet factor 4 (PF4 or CXCL4) and the erythrocyte Duffy-antigen receptor (Fy) are necessary for platelet-mediated killing of Plasmodium falciparum parasites. PF4 is released by platelets on contact with parasitized red cells, and the protein directly kills intraerythrocytic parasites. This function for PF4 is critically dependent on Fy, which binds PF4. Genetic disruption of Fy expression inhibits binding of PF4 to parasitized cells and concomitantly prevents parasite killing by both human platelets and recombinant human PF4. The protective function afforded by platelets during a malarial infection may therefore be compromised in Duffy-negative individuals, who do not express Fy.

Full Text

Duke Authors

Cited Authors

  • McMorran, BJ; Wieczorski, L; Drysdale, KE; Chan, J-A; Huang, HM; Smith, C; Mitiku, C; Beeson, JG; Burgio, G; Foote, SJ

Published Date

  • December 7, 2012

Published In

Volume / Issue

  • 338 / 6112

Start / End Page

  • 1348 - 1351

PubMed ID

  • 23224555

Pubmed Central ID

  • 23224555

Electronic International Standard Serial Number (EISSN)

  • 1095-9203

Digital Object Identifier (DOI)

  • 10.1126/science.1228892

Language

  • eng

Conference Location

  • United States