Continuous focal translation enhances rate of point-scan volumetric microscopy.
Published
Journal Article
Two-Photon Laser-Scanning Microscopy is a powerful tool for exploring biological structure and function due to its ability to optically section through a sample with a tight focus. While it is possible to obtain 3D image stacks by moving a stage, this per-frame imaging process is time consuming. Here, we present a method for an easy-to-implement and inexpensive modification of an existing two-photon microscope to rapidly image in 3D using an electrically tunable lens to create a tessellating scan pattern which repeats with the volume rate. Using appropriate interpolating algorithms, the volumetric imaging rate can be increased by a factor up to four-fold. This capability provides the expansion of the two-photon microscope into the third dimension for faster volumetric imaging capable of visualizing dynamics on timescales not achievable by traditional stage-stack methods.
Full Text
Duke Authors
Cited Authors
- Johnson, C; Exell, J; Kuo, J; Welsher, K
Published Date
- December 2019
Published In
Volume / Issue
- 27 / 25
Start / End Page
- 36241 - 36258
PubMed ID
- 31873407
Pubmed Central ID
- 31873407
Electronic International Standard Serial Number (EISSN)
- 1094-4087
International Standard Serial Number (ISSN)
- 1094-4087
Digital Object Identifier (DOI)
- 10.1364/oe.27.036241
Language
- eng