Modulating Macrophage Phenotype by Sustained MicroRNA Delivery Improves Host-Implant Integration.

Journal Article (Journal Article)

Biomedical implant failure due to the host's response remains a challenging problem. In particular, the formation of the fibrous capsule is a common barrier for the normal function of implants. Currently, there is mounting evidence indicating that the polarization state of macrophages plays an important role in effecting the foreign body reaction (FBR). This opens up a potential avenue for improving host-implant integration. Here, electrospun poly(caprolactone-co-ethyl ethylene phosphate) nanofiber scaffolds are utilized to deliver microRNAs (miRs) to induce macrophage polarization and modulate FBR. Specifically, C57BL/6 mice that are treated with M2-inducing miRs, Let-7c and miR-124, display relatively thinner fibrous capsule formation around the scaffolds at both Week 2 and 4, as compared to treatment with M1-inducing miR, Anti-Let-7c. Histological analysis shows that the density of blood vessels in the scaffolds are the highest in miR-124 treatment group, followed by Anti-Let-7c and Let-7c treatment groups. Based on immunohistochemical quantifications, these miR-encapsulated nanofiber scaffolds are useful for localized and sustained delivery of functional miRs and are able to modulate macrophage polarization during the first 2 weeks of implantation to result in significant alteration in host-implant integration at longer time points.

Full Text

Duke Authors

Cited Authors

  • Lin, J; Mohamed, I; Lin, PH; Shirahama, H; Milbreta, U; Sieow, JL; Peng, Y; Bugiani, M; Wong, SC; Levinson, H; Chew, SY

Published Date

  • February 2020

Published In

Volume / Issue

  • 9 / 3

Start / End Page

  • e1901257 -

PubMed ID

  • 31854130

Electronic International Standard Serial Number (EISSN)

  • 2192-2659

International Standard Serial Number (ISSN)

  • 2192-2640

Digital Object Identifier (DOI)

  • 10.1002/adhm.201901257

Language

  • eng