Inhibition of endoglin-GIPC interaction inhibits pancreatic cancer cell growth.

Published

Journal Article

Endoglin, a 180-kDa disulfide-linked homodimeric transmembrane receptor protein mostly expressed in tumor-associated endothelial cells, is an endogenous binding partner of GAIP-interacting protein, C terminus (GIPC). Endoglin functions as a coreceptor of TβRII that binds TGFβ and is important for vascular development, and consequently has become a compelling target for antiangiogenic therapies. A few recent studies in gastrointestinal stromal tumor (GIST), breast cancer, and ovarian cancer, however, suggest that endoglin is upregulated in tumor cells and is associated with poor prognosis. These findings indicate a broader role of endoglin in tumor biology, beyond angiogenic effects. The goal of our current study is to evaluate the effects of targeting endoglin in pancreatic cancer both in vitro and in vivo. We analyzed the antiproliferative effect of both RNAi-based and peptide ligand-based inhibition of endoglin in pancreatic cancer cell lines, the latter yielding a GIPC PDZ domain-targeting lipopeptide with notable antiproliferative activity. We further demonstrated that endoglin inhibition induced a differentiation phenotype in the pancreatic cancer cells and sensitized them against conventional chemotherapeutic drug gemcitabine. Most importantly, we have demonstrated the antitumor effect of both RNAi-based and competitive inhibitor-based blocking of endoglin in pancreatic cancer xenograft models in vivo. To our knowledge, this is the first report exploring the effect of targeting endoglin in pancreatic cancer cells.

Full Text

Duke Authors

Cited Authors

  • Pal, K; Pletnev, AA; Dutta, SK; Wang, E; Zhao, R; Baral, A; Yadav, VK; Aggarwal, S; Krishnaswamy, S; Alkharfy, KM; Chowdhury, S; Spaller, MR; Mukhopadhyay, D

Published Date

  • October 2014

Published In

Volume / Issue

  • 13 / 10

Start / End Page

  • 2264 - 2275

PubMed ID

  • 25125675

Pubmed Central ID

  • 25125675

Electronic International Standard Serial Number (EISSN)

  • 1538-8514

International Standard Serial Number (ISSN)

  • 1535-7163

Digital Object Identifier (DOI)

  • 10.1158/1535-7163.mct-14-0291

Language

  • eng