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Chromogenic assay for phospholipase C from Bacillus cereus.

Publication ,  Journal Article
Hergenrother, PJ; Spaller, MR; Haas, MK; Martin, SF
Published in: Analytical biochemistry
August 1995

A standard chromogenic assay for phosphatidylcholine determination has been modified in the development of a facile method for measuring the rate parameters (Km and Vmax) for phosphatidylcholine-preferring phospholipase C from Bacillus cereus (PLCBc). The assay is applied to the determination of the Ki for a known inhibitor of PLCBc.

Duke Scholars

Published In

Analytical biochemistry

DOI

EISSN

1096-0309

ISSN

0003-2697

Publication Date

August 1995

Volume

229

Issue

2

Start / End Page

313 / 316

Related Subject Headings

  • Type C Phospholipases
  • Time Factors
  • Substrate Specificity
  • Spectrophotometry
  • Phosphatidylcholines
  • Kinetics
  • Indicators and Reagents
  • Biochemistry & Molecular Biology
  • Bacillus cereus
  • 3401 Analytical chemistry
 

Citation

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MLA
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Hergenrother, P. J., Spaller, M. R., Haas, M. K., & Martin, S. F. (1995). Chromogenic assay for phospholipase C from Bacillus cereus. Analytical Biochemistry, 229(2), 313–316. https://doi.org/10.1006/abio.1995.1418
Hergenrother, P. J., M. R. Spaller, M. K. Haas, and S. F. Martin. “Chromogenic assay for phospholipase C from Bacillus cereus.Analytical Biochemistry 229, no. 2 (August 1995): 313–16. https://doi.org/10.1006/abio.1995.1418.
Hergenrother PJ, Spaller MR, Haas MK, Martin SF. Chromogenic assay for phospholipase C from Bacillus cereus. Analytical biochemistry. 1995 Aug;229(2):313–6.
Hergenrother, P. J., et al. “Chromogenic assay for phospholipase C from Bacillus cereus.Analytical Biochemistry, vol. 229, no. 2, Aug. 1995, pp. 313–16. Epmc, doi:10.1006/abio.1995.1418.
Hergenrother PJ, Spaller MR, Haas MK, Martin SF. Chromogenic assay for phospholipase C from Bacillus cereus. Analytical biochemistry. 1995 Aug;229(2):313–316.
Journal cover image

Published In

Analytical biochemistry

DOI

EISSN

1096-0309

ISSN

0003-2697

Publication Date

August 1995

Volume

229

Issue

2

Start / End Page

313 / 316

Related Subject Headings

  • Type C Phospholipases
  • Time Factors
  • Substrate Specificity
  • Spectrophotometry
  • Phosphatidylcholines
  • Kinetics
  • Indicators and Reagents
  • Biochemistry & Molecular Biology
  • Bacillus cereus
  • 3401 Analytical chemistry