Chromogenic assay for phospholipase C from Bacillus cereus.
Publication
, Journal Article
Hergenrother, PJ; Spaller, MR; Haas, MK; Martin, SF
Published in: Analytical biochemistry
August 1995
A standard chromogenic assay for phosphatidylcholine determination has been modified in the development of a facile method for measuring the rate parameters (Km and Vmax) for phosphatidylcholine-preferring phospholipase C from Bacillus cereus (PLCBc). The assay is applied to the determination of the Ki for a known inhibitor of PLCBc.
Duke Scholars
Published In
Analytical biochemistry
DOI
EISSN
1096-0309
ISSN
0003-2697
Publication Date
August 1995
Volume
229
Issue
2
Start / End Page
313 / 316
Related Subject Headings
- Type C Phospholipases
- Time Factors
- Substrate Specificity
- Spectrophotometry
- Phosphatidylcholines
- Kinetics
- Indicators and Reagents
- Biochemistry & Molecular Biology
- Bacillus cereus
- 3401 Analytical chemistry
Citation
APA
Chicago
ICMJE
MLA
NLM
Hergenrother, P. J., Spaller, M. R., Haas, M. K., & Martin, S. F. (1995). Chromogenic assay for phospholipase C from Bacillus cereus. Analytical Biochemistry, 229(2), 313–316. https://doi.org/10.1006/abio.1995.1418
Hergenrother, P. J., M. R. Spaller, M. K. Haas, and S. F. Martin. “Chromogenic assay for phospholipase C from Bacillus cereus.” Analytical Biochemistry 229, no. 2 (August 1995): 313–16. https://doi.org/10.1006/abio.1995.1418.
Hergenrother PJ, Spaller MR, Haas MK, Martin SF. Chromogenic assay for phospholipase C from Bacillus cereus. Analytical biochemistry. 1995 Aug;229(2):313–6.
Hergenrother, P. J., et al. “Chromogenic assay for phospholipase C from Bacillus cereus.” Analytical Biochemistry, vol. 229, no. 2, Aug. 1995, pp. 313–16. Epmc, doi:10.1006/abio.1995.1418.
Hergenrother PJ, Spaller MR, Haas MK, Martin SF. Chromogenic assay for phospholipase C from Bacillus cereus. Analytical biochemistry. 1995 Aug;229(2):313–316.
Published In
Analytical biochemistry
DOI
EISSN
1096-0309
ISSN
0003-2697
Publication Date
August 1995
Volume
229
Issue
2
Start / End Page
313 / 316
Related Subject Headings
- Type C Phospholipases
- Time Factors
- Substrate Specificity
- Spectrophotometry
- Phosphatidylcholines
- Kinetics
- Indicators and Reagents
- Biochemistry & Molecular Biology
- Bacillus cereus
- 3401 Analytical chemistry