Chromogenic assay for phospholipase C from Bacillus cereus.
Journal Article (Journal Article)
A standard chromogenic assay for phosphatidylcholine determination has been modified in the development of a facile method for measuring the rate parameters (Km and Vmax) for phosphatidylcholine-preferring phospholipase C from Bacillus cereus (PLCBc). The assay is applied to the determination of the Ki for a known inhibitor of PLCBc.
Full Text
Duke Authors
Cited Authors
- Hergenrother, PJ; Spaller, MR; Haas, MK; Martin, SF
Published Date
- August 1995
Published In
Volume / Issue
- 229 / 2
Start / End Page
- 313 - 316
PubMed ID
- 7485988
Electronic International Standard Serial Number (EISSN)
- 1096-0309
International Standard Serial Number (ISSN)
- 0003-2697
Digital Object Identifier (DOI)
- 10.1006/abio.1995.1418
Language
- eng