Chromogenic assay for phospholipase C from Bacillus cereus.

Journal Article (Journal Article)

A standard chromogenic assay for phosphatidylcholine determination has been modified in the development of a facile method for measuring the rate parameters (Km and Vmax) for phosphatidylcholine-preferring phospholipase C from Bacillus cereus (PLCBc). The assay is applied to the determination of the Ki for a known inhibitor of PLCBc.

Full Text

Published version (via Digital Object Identifier)

Duke Authors

Spaller, Mark Robert

Cited Authors

Hergenrother, PJ; Spaller, MR; Haas, MK; Martin, SF

Published Date

August 1995

Published In

Analytical Biochemistry

Volume / Issue

229 / 2

Start / End Page

313 - 316

PubMed ID

7485988

Electronic International Standard Serial Number (EISSN)

1096-0309

International Standard Serial Number (ISSN)

0003-2697

Digital Object Identifier (DOI)

10.1006/abio.1995.1418

Language

eng