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Tyrosine-kinase-dependent recruitment of RGS12 to the N-type calcium channel.

Publication ,  Journal Article
Schiff, ML; Siderovski, DP; Jordan, JD; Brothers, G; Snow, B; De Vries, L; Ortiz, DF; Diversé-Pierluissi, M
Published in: Nature
December 7, 2000

Gamma-aminobutyric acid (GABA)B receptors couple to Go to inhibit N-type calcium channels in embryonic chick dorsal root ganglion neurons. The voltage-independent inhibition, mediated by means of a tyrosine-kinase pathway, is transient and lasts up to 100 seconds. Inhibition of endogenous RGS12, a member of the family of regulators of G-protein signalling, selectively alters the time course of voltage-independent inhibition. The RGS12 protein, in addition to the RGS domain, contains PDZ and PTB domains. Fusion proteins containing the PTB domain of RGS12 alter the rate of termination of the GABA(B) signal, whereas the PDZ or RGS domains of RGS 12 have no observable effects. Using primary dorsal root ganglion neurons in culture, here we show an endogenous agonist-induced tyrosine-kinase-dependent complex of RGS12 and the calcium channel. These results indicate that RGS12 is a multifunctional protein capable of direct interactions through its PTB domain with the tyrosine-phosphorylated calcium channel. Recruitment of RGS proteins to G-protein effectors may represent an additional mechanism for signal termination in G-protein-coupled pathways.

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Published In

Nature

DOI

ISSN

0028-0836

Publication Date

December 7, 2000

Volume

408

Issue

6813

Start / End Page

723 / 727

Location

England

Related Subject Headings

  • Signal Transduction
  • Recombinant Fusion Proteins
  • Receptors, GABA-B
  • Rats
  • RGS Proteins
  • Protein-Tyrosine Kinases
  • Protein Structure, Tertiary
  • Phosphorylation
  • Neurons
  • Molecular Sequence Data
 

Citation

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Schiff, M. L., Siderovski, D. P., Jordan, J. D., Brothers, G., Snow, B., De Vries, L., … Diversé-Pierluissi, M. (2000). Tyrosine-kinase-dependent recruitment of RGS12 to the N-type calcium channel. Nature, 408(6813), 723–727. https://doi.org/10.1038/35047093
Schiff, M. L., D. P. Siderovski, J. D. Jordan, G. Brothers, B. Snow, L. De Vries, D. F. Ortiz, and M. Diversé-Pierluissi. “Tyrosine-kinase-dependent recruitment of RGS12 to the N-type calcium channel.Nature 408, no. 6813 (December 7, 2000): 723–27. https://doi.org/10.1038/35047093.
Schiff ML, Siderovski DP, Jordan JD, Brothers G, Snow B, De Vries L, et al. Tyrosine-kinase-dependent recruitment of RGS12 to the N-type calcium channel. Nature. 2000 Dec 7;408(6813):723–7.
Schiff, M. L., et al. “Tyrosine-kinase-dependent recruitment of RGS12 to the N-type calcium channel.Nature, vol. 408, no. 6813, Dec. 2000, pp. 723–27. Pubmed, doi:10.1038/35047093.
Schiff ML, Siderovski DP, Jordan JD, Brothers G, Snow B, De Vries L, Ortiz DF, Diversé-Pierluissi M. Tyrosine-kinase-dependent recruitment of RGS12 to the N-type calcium channel. Nature. 2000 Dec 7;408(6813):723–727.
Journal cover image

Published In

Nature

DOI

ISSN

0028-0836

Publication Date

December 7, 2000

Volume

408

Issue

6813

Start / End Page

723 / 727

Location

England

Related Subject Headings

  • Signal Transduction
  • Recombinant Fusion Proteins
  • Receptors, GABA-B
  • Rats
  • RGS Proteins
  • Protein-Tyrosine Kinases
  • Protein Structure, Tertiary
  • Phosphorylation
  • Neurons
  • Molecular Sequence Data