HPV-16-related DNA sequences in Kaposi's sarcoma.

Journal Article (Journal Article)

In the USA, Kaposi's sarcoma associated with the acquired immunodeficiency syndrome (AIDS-KS) is ten times more common in homosexual or bisexual men than in heterosexual men with AIDS. One explanation for this finding is that AIDS-KS may be caused by an infectious agent. Because there is a high incidence of human papillomavirus (HPV) infection, especially HPV-16, in homosexual men, we have sought HPV DNA sequences in Kaposi's sarcoma. We used the polymerase chain reaction with a primer pair specific for the highly conserved E6 region of HPV-16 to detect HPV-16 homologous DNA fragments in tumour tissues from 97 patients with KS and in KS-derived cell cultures. HPV DNA sequences were found in 11 of 69 KS skin tumours from homosexual men with AIDS-KS, in 3 of 11 KS biopsy specimens from homosexual men who had no clinical or laboratory evidence of HIV-infection, and in 5 of 17 KS skin lesions from HIV-1-negative elderly men and women with classic KS. The same primer pair amplified HPV-16 homologous fragments from two different continuous cell cultures derived from pleural effusion fluid of patients with pulmonary AIDS-KS and two continuous cell cultures derived from KS skin lesions. The findings suggest that HPV-16-related DNA sequences are associated with different forms of KS and may have a role in the pathogenesis of this neoplasm.

Full Text

Duke Authors

Cited Authors

  • Huang, YQ; Li, JJ; Rush, MG; Poiesz, BJ; Nicolaides, A; Jacobson, M; Zhang, WG; Coutavas, E; Abbott, MA; Friedman-Kien, AE

Published Date

  • February 29, 1992

Published In

Volume / Issue

  • 339 / 8792

Start / End Page

  • 515 - 518

PubMed ID

  • 1346878

International Standard Serial Number (ISSN)

  • 0140-6736

Digital Object Identifier (DOI)

  • 10.1016/0140-6736(92)90338-4

Language

  • eng

Conference Location

  • England