Characterization of proteins that interact with the cell-cycle regulatory protein Ran/TC4.

Journal Article (Journal Article)

The human Ras-related nuclear protein Ran/TC4 (refs 1-4) is the prototype of a well conserved family of GTPases that can regulate both cell-cycle progression and messenger RNA transport. Ran has been proposed to undergo tightly controlled cycles of GTP binding and hydrolysis, to operate as a GTPase switch whose GTP- and GDP-bound forms interact differentially with regulators and effectors. One known regulator, the protein RCC1 (refs 12, 13), interacts with Ran to catalyse guanine nucleotide exchange, and both RCC1 and Ran are components of an intrinsic checkpoint control that prevents the premature initiation of mitosis. To test and extend the GTPase-switch model, we searched for a Ran-specific GTPase-activating protein (GAP), and for putative effectors (proteins that interact specifically with Ran/TC4-GTP). We report here the identification of a Ran GAP and its use to characterize the GTP-hydrolysing properties of mutant Ran proteins, and the identification and cloning of a binding protein specific for Ran/TC4-GTP.

Full Text

Duke Authors

Cited Authors

  • Coutavas, E; Ren, M; Oppenheim, JD; D'Eustachio, P; Rush, MG

Published Date

  • December 9, 1993

Published In

Volume / Issue

  • 366 / 6455

Start / End Page

  • 585 - 587

PubMed ID

  • 8255297

International Standard Serial Number (ISSN)

  • 0028-0836

Digital Object Identifier (DOI)

  • 10.1038/366585a0


  • eng

Conference Location

  • England