Outer Membrane Vesiculation Facilitates Surface Exchange and In Vivo Adaptation of Vibrio cholerae.

Journal Article (Journal Article)

Gram-negative bacteria release outer membrane vesicles into the external milieu to deliver effector molecules that alter the host and facilitate virulence. Vesicle formation is driven by phospholipid accumulation in the outer membrane and regulated by the phospholipid transporter VacJ/Yrb. We use the facultative human pathogen Vibrio cholerae to show that VacJ/Yrb is silenced early during mammalian infection, which stimulates vesiculation that expedites bacterial surface exchange and adaptation to the host environment. Hypervesiculating strains rapidly alter their bacterial membrane composition and exhibit enhanced intestinal colonization fitness. This adaptation is exemplified by faster accumulation of glycine-modified lipopolysaccharide (LPS) and depletion of outer membrane porin OmpT, which confers resistance to host-derived antimicrobial peptides and bile, respectively. The competitive advantage of hypervesiculation is lost upon pre-adaptation to bile and antimicrobial peptides, indicating the importance of these adaptive processes. Thus, bacteria use outer membrane vesiculation to exchange cell surface components, thereby increasing survival during mammalian infection.

Full Text

Duke Authors

Cited Authors

  • Zingl, FG; Kohl, P; Cakar, F; Leitner, DR; Mitterer, F; Bonnington, KE; Rechberger, GN; Kuehn, MJ; Guan, Z; Reidl, J; Schild, S

Published Date

  • February 12, 2020

Published In

Volume / Issue

  • 27 / 2

Start / End Page

  • 225 - 237.e8

PubMed ID

  • 31901519

Pubmed Central ID

  • PMC7155939

Electronic International Standard Serial Number (EISSN)

  • 1934-6069

Digital Object Identifier (DOI)

  • 10.1016/j.chom.2019.12.002

Language

  • eng

Conference Location

  • United States