Skip to main content
Journal cover image

Actin is required for IFT regulation in Chlamydomonas reinhardtii.

Publication ,  Journal Article
Avasthi, P; Onishi, M; Karpiak, J; Yamamoto, R; Mackinder, L; Jonikas, MC; Sale, WS; Shoichet, B; Pringle, JR; Marshall, WF
Published in: Current biology : CB
September 2014

Assembly of cilia and flagella requires intraflagellar transport (IFT), a highly regulated kinesin-based transport system that moves cargo from the basal body to the tip of flagella [1]. The recruitment of IFT components to basal bodies is a function of flagellar length, with increased recruitment in rapidly growing short flagella [2]. The molecular pathways regulating IFT are largely a mystery. Because actin network disruption leads to changes in ciliary length and number, actin has been proposed to have a role in ciliary assembly. However, the mechanisms involved are unknown. In Chlamydomonas reinhardtii, conventional actin is found in both the cell body and the inner dynein arm complexes within flagella [3, 4]. Previous work showed that treating Chlamydomonas cells with the actin-depolymerizing compound cytochalasin D resulted in reversible flagellar shortening [5], but how actin is related to flagellar length or assembly remains unknown. Here we utilize small-molecule inhibitors and genetic mutants to analyze the role of actin dynamics in flagellar assembly in Chlamydomonas reinhardtii. We demonstrate that actin plays a role in IFT recruitment to basal bodies during flagellar elongation and that when actin is perturbed, the normal dependence of IFT recruitment on flagellar length is lost. We also find that actin is required for sufficient entry of IFT material into flagella during assembly. These same effects are recapitulated with a myosin inhibitor, suggesting that actin may act via myosin in a pathway by which flagellar assembly is regulated by flagellar length.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Current biology : CB

DOI

EISSN

1879-0445

ISSN

0960-9822

Publication Date

September 2014

Volume

24

Issue

17

Start / End Page

2025 / 2032

Related Subject Headings

  • Protein Multimerization
  • Intracellular Signaling Peptides and Proteins
  • Flagella
  • Developmental Biology
  • Chlamydomonas reinhardtii
  • Biological Transport
  • Axoneme
  • Actins
  • 52 Psychology
  • 32 Biomedical and clinical sciences
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Avasthi, P., Onishi, M., Karpiak, J., Yamamoto, R., Mackinder, L., Jonikas, M. C., … Marshall, W. F. (2014). Actin is required for IFT regulation in Chlamydomonas reinhardtii. Current Biology : CB, 24(17), 2025–2032. https://doi.org/10.1016/j.cub.2014.07.038
Avasthi, Prachee, Masayuki Onishi, Joel Karpiak, Ryosuke Yamamoto, Luke Mackinder, Martin C. Jonikas, Winfield S. Sale, Brian Shoichet, John R. Pringle, and Wallace F. Marshall. “Actin is required for IFT regulation in Chlamydomonas reinhardtii.Current Biology : CB 24, no. 17 (September 2014): 2025–32. https://doi.org/10.1016/j.cub.2014.07.038.
Avasthi P, Onishi M, Karpiak J, Yamamoto R, Mackinder L, Jonikas MC, et al. Actin is required for IFT regulation in Chlamydomonas reinhardtii. Current biology : CB. 2014 Sep;24(17):2025–32.
Avasthi, Prachee, et al. “Actin is required for IFT regulation in Chlamydomonas reinhardtii.Current Biology : CB, vol. 24, no. 17, Sept. 2014, pp. 2025–32. Epmc, doi:10.1016/j.cub.2014.07.038.
Avasthi P, Onishi M, Karpiak J, Yamamoto R, Mackinder L, Jonikas MC, Sale WS, Shoichet B, Pringle JR, Marshall WF. Actin is required for IFT regulation in Chlamydomonas reinhardtii. Current biology : CB. 2014 Sep;24(17):2025–2032.
Journal cover image

Published In

Current biology : CB

DOI

EISSN

1879-0445

ISSN

0960-9822

Publication Date

September 2014

Volume

24

Issue

17

Start / End Page

2025 / 2032

Related Subject Headings

  • Protein Multimerization
  • Intracellular Signaling Peptides and Proteins
  • Flagella
  • Developmental Biology
  • Chlamydomonas reinhardtii
  • Biological Transport
  • Axoneme
  • Actins
  • 52 Psychology
  • 32 Biomedical and clinical sciences