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Fission yeast Cyk3p is a transglutaminase-like protein that participates in cytokinesis and cell morphogenesis.

Publication ,  Journal Article
Pollard, LW; Onishi, M; Pringle, JR; Lord, M
Published in: Molecular biology of the cell
July 2012

Cell morphogenesis is a complex process that relies on a diverse array of proteins and pathways. We have identified a transglutaminase-like protein (Cyk3p) that functions in fission yeast morphogenesis. The phenotype of a cyk3 knockout strain indicates a primary role for Cyk3p in cytokinesis. Correspondingly, Cyk3p localizes both to the actomyosin contractile ring and the division septum, promoting ring constriction, septation, and subsequent cell separation following ring disassembly. In addition, Cyk3p localizes to polarized growth sites and plays a role in cell shape determination, and it also appears to contribute to cell integrity during stationary phase, given its accumulation as dynamic puncta at the cortex of such cells. Our results and the conservation of Cyk3p across fungi point to a role in cell wall synthesis and remodeling. Cyk3p possesses a transglutaminase domain that is essential for function, even though it lacks the catalytic active site. In a wider sense, our work illustrates the physiological importance of inactive members of the transglutaminase family, which are found throughout eukaryotes. We suggest that the proposed evolution of animal transglutaminase cross-linking activity from ancestral bacterial thiol proteases was accompanied by the emergence of a subclass whose function does not depend on enzymatic activity.

Duke Scholars

Published In

Molecular biology of the cell

DOI

EISSN

1939-4586

ISSN

1059-1524

Publication Date

July 2012

Volume

23

Issue

13

Start / End Page

2433 / 2444

Related Subject Headings

  • Time-Lapse Imaging
  • Spindle Apparatus
  • Sequence Deletion
  • Schizosaccharomyces pombe Proteins
  • Schizosaccharomyces
  • Recombinant Fusion Proteins
  • Protein Transport
  • Protein Structure, Tertiary
  • Morphogenesis
  • Kinetics
 

Citation

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MLA
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Pollard, L. W., Onishi, M., Pringle, J. R., & Lord, M. (2012). Fission yeast Cyk3p is a transglutaminase-like protein that participates in cytokinesis and cell morphogenesis. Molecular Biology of the Cell, 23(13), 2433–2444. https://doi.org/10.1091/mbc.e11-07-0656
Pollard, Luther W., Masayuki Onishi, John R. Pringle, and Matthew Lord. “Fission yeast Cyk3p is a transglutaminase-like protein that participates in cytokinesis and cell morphogenesis.Molecular Biology of the Cell 23, no. 13 (July 2012): 2433–44. https://doi.org/10.1091/mbc.e11-07-0656.
Pollard LW, Onishi M, Pringle JR, Lord M. Fission yeast Cyk3p is a transglutaminase-like protein that participates in cytokinesis and cell morphogenesis. Molecular biology of the cell. 2012 Jul;23(13):2433–44.
Pollard, Luther W., et al. “Fission yeast Cyk3p is a transglutaminase-like protein that participates in cytokinesis and cell morphogenesis.Molecular Biology of the Cell, vol. 23, no. 13, July 2012, pp. 2433–44. Epmc, doi:10.1091/mbc.e11-07-0656.
Pollard LW, Onishi M, Pringle JR, Lord M. Fission yeast Cyk3p is a transglutaminase-like protein that participates in cytokinesis and cell morphogenesis. Molecular biology of the cell. 2012 Jul;23(13):2433–2444.

Published In

Molecular biology of the cell

DOI

EISSN

1939-4586

ISSN

1059-1524

Publication Date

July 2012

Volume

23

Issue

13

Start / End Page

2433 / 2444

Related Subject Headings

  • Time-Lapse Imaging
  • Spindle Apparatus
  • Sequence Deletion
  • Schizosaccharomyces pombe Proteins
  • Schizosaccharomyces
  • Recombinant Fusion Proteins
  • Protein Transport
  • Protein Structure, Tertiary
  • Morphogenesis
  • Kinetics