Characterization of vps33+, a gene required for vacuolar biogenesis and protein sorting in Schizosaccharomyces pombe.

Published

Journal Article

From the fission yeast Schizosaccharomyces pombe we have identified and deleted vps33, a gene encoding a homologue of VPS33, which is required for vacuolar biogenesis in S. cerevisiae cells. When the vps33(+) gene is disrupted, Sz. pombe strains are temperature-sensitive for growth and contain numerous small vesicular structures stained with FM4-64 in the cells. Deletion of the Sz. pombe vps33(+) gene results in pleiotropic phenotypes consistent with the absence of normal vacuoles, including missorting of vacuolar carboxypeptidase Y, various ion- and drug-sensitivities, and sporulation defects. These results are consistent with Vps33p being necessary for the morphogenesis of vacuoles and subsequent expression of vacuolar functions in Sz. pombe cells.

Full Text

Duke Authors

Cited Authors

  • Iwaki, T; Osawa, F; Onishi, M; Koga, T; Fujita, Y; Hosomi, A; Tanaka, N; Fukui, Y; Takegawa, K

Published Date

  • July 2003

Published In

Volume / Issue

  • 20 / 10

Start / End Page

  • 845 - 855

PubMed ID

  • 12868054

Pubmed Central ID

  • 12868054

Electronic International Standard Serial Number (EISSN)

  • 1097-0061

International Standard Serial Number (ISSN)

  • 0749-503X

Digital Object Identifier (DOI)

  • 10.1002/yea.1011

Language

  • eng