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Role of septins in the orientation of forespore membrane extension during sporulation in fission yeast.

Publication ,  Journal Article
Onishi, M; Koga, T; Hirata, A; Nakamura, T; Asakawa, H; Shimoda, C; Bähler, J; Wu, J-Q; Takegawa, K; Tachikawa, H; Pringle, JR; Fukui, Y
Published in: Molecular and cellular biology
April 2010

During yeast sporulation, a forespore membrane (FSM) initiates at each spindle-pole body and extends to form the spore envelope. We used Schizosaccharomyces pombe to investigate the role of septins during this process. During the prior conjugation of haploid cells, the four vegetatively expressed septins (Spn1, Spn2, Spn3, and Spn4) coassemble at the fusion site and are necessary for its normal morphogenesis. Sporulation involves a different set of four septins (Spn2, Spn5, Spn6, and the atypical Spn7) that does not include the core subunits of the vegetative septin complex. The four sporulation septins form a complex in vitro and colocalize interdependently to a ring-shaped structure along each FSM, and septin mutations result in disoriented FSM extension. The septins and the leading-edge proteins appear to function in parallel to orient FSM extension. Spn2 and Spn7 bind to phosphatidylinositol 4-phosphate [PtdIns(4)P] in vitro, and PtdIns(4)P is enriched in the FSMs, suggesting that septins bind to the FSMs via this lipid. Cells expressing a mutant Spn2 protein unable to bind PtdIns(4)P still form extended septin structures, but these structures fail to associate with the FSMs, which are frequently disoriented. Thus, septins appear to form a scaffold that helps to guide the oriented extension of the FSM.

Duke Scholars

Published In

Molecular and cellular biology

DOI

EISSN

1098-5549

ISSN

0270-7306

Publication Date

April 2010

Volume

30

Issue

8

Start / End Page

2057 / 2074

Related Subject Headings

  • Spores, Fungal
  • Sequence Alignment
  • Schizosaccharomyces pombe Proteins
  • Schizosaccharomyces
  • Phosphatidylinositol Phosphates
  • Mutation
  • Molecular Sequence Data
  • GTP-Binding Proteins
  • Developmental Biology
  • Cell Surface Extensions
 

Citation

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Onishi, M., Koga, T., Hirata, A., Nakamura, T., Asakawa, H., Shimoda, C., … Fukui, Y. (2010). Role of septins in the orientation of forespore membrane extension during sporulation in fission yeast. Molecular and Cellular Biology, 30(8), 2057–2074. https://doi.org/10.1128/mcb.01529-09
Onishi, Masayuki, Takako Koga, Aiko Hirata, Taro Nakamura, Haruhiko Asakawa, Chikashi Shimoda, Jürg Bähler, et al. “Role of septins in the orientation of forespore membrane extension during sporulation in fission yeast.Molecular and Cellular Biology 30, no. 8 (April 2010): 2057–74. https://doi.org/10.1128/mcb.01529-09.
Onishi M, Koga T, Hirata A, Nakamura T, Asakawa H, Shimoda C, et al. Role of septins in the orientation of forespore membrane extension during sporulation in fission yeast. Molecular and cellular biology. 2010 Apr;30(8):2057–74.
Onishi, Masayuki, et al. “Role of septins in the orientation of forespore membrane extension during sporulation in fission yeast.Molecular and Cellular Biology, vol. 30, no. 8, Apr. 2010, pp. 2057–74. Epmc, doi:10.1128/mcb.01529-09.
Onishi M, Koga T, Hirata A, Nakamura T, Asakawa H, Shimoda C, Bähler J, Wu J-Q, Takegawa K, Tachikawa H, Pringle JR, Fukui Y. Role of septins in the orientation of forespore membrane extension during sporulation in fission yeast. Molecular and cellular biology. 2010 Apr;30(8):2057–2074.

Published In

Molecular and cellular biology

DOI

EISSN

1098-5549

ISSN

0270-7306

Publication Date

April 2010

Volume

30

Issue

8

Start / End Page

2057 / 2074

Related Subject Headings

  • Spores, Fungal
  • Sequence Alignment
  • Schizosaccharomyces pombe Proteins
  • Schizosaccharomyces
  • Phosphatidylinositol Phosphates
  • Mutation
  • Molecular Sequence Data
  • GTP-Binding Proteins
  • Developmental Biology
  • Cell Surface Extensions