Load-dependent release limits the processive stepping of the tetrameric Eg5 motor.

Journal Article (Journal Article)

Tetrameric motor proteins of the Kinesin-5 family are essential for eukaryotic cell division. The microscopic mechanism by which Eg5, the vertebrate Kinesin-5, drives bipolar mitotic spindle formation remains unknown. Here we show in optical trapping experiments that full-length Eg5 moves processively and stepwise along microtubule bundles. Interestingly, the force produced by individual Eg5 motors typically reached only approximately 2 pN, one-third of the stall force of Kinesin-1. Eg5 typically detached from microtubules before stalling. This behavior may reflect a regulatory mechanism important for the role of Eg5 in the mitotic spindle.

Full Text

Duke Authors

Cited Authors

  • Korneev, MJ; Lakämper, S; Schmidt, CF

Published Date

  • July 2007

Published In

Volume / Issue

  • 36 / 6

Start / End Page

  • 675 - 681

PubMed ID

  • 17333163

Pubmed Central ID

  • PMC1914257

Electronic International Standard Serial Number (EISSN)

  • 1432-1017

International Standard Serial Number (ISSN)

  • 0175-7571

Digital Object Identifier (DOI)

  • 10.1007/s00249-007-0134-6


  • eng