Direct observation of kinesin stepping by optical trapping interferometry.

Journal Article (Journal Article)

Do biological motors move with regular steps? To address this question, we constructed instrumentation with the spatial and temporal sensitivity to resolve movement on a molecular scale. We deposited silica beads carrying single molecules of the motor protein kinesin on microtubules using optical tweezers and analysed their motion under controlled loads by interferometry. We find that kinesin moves with 8-nm steps.

Full Text

Duke Authors

Cited Authors

  • Svoboda, K; Schmidt, CF; Schnapp, BJ; Block, SM

Published Date

  • October 1993

Published In

Volume / Issue

  • 365 / 6448

Start / End Page

  • 721 - 727

PubMed ID

  • 8413650

Electronic International Standard Serial Number (EISSN)

  • 1476-4687

International Standard Serial Number (ISSN)

  • 0028-0836

Digital Object Identifier (DOI)

  • 10.1038/365721a0


  • eng