Direct observation of kinesin stepping by optical trapping interferometry.

Journal Article (Journal Article)

Do biological motors move with regular steps? To address this question, we constructed instrumentation with the spatial and temporal sensitivity to resolve movement on a molecular scale. We deposited silica beads carrying single molecules of the motor protein kinesin on microtubules using optical tweezers and analysed their motion under controlled loads by interferometry. We find that kinesin moves with 8-nm steps.

Full Text

Published version (via Digital Object Identifier)

Duke Authors

Schmidt, Christoph F.

Cited Authors

Svoboda, K; Schmidt, CF; Schnapp, BJ; Block, SM

Published Date

October 1993

Published In

Nature

Volume / Issue

365 / 6448

Start / End Page

721 - 727

PubMed ID

8413650

Electronic International Standard Serial Number (EISSN)

1476-4687

International Standard Serial Number (ISSN)

0028-0836

Digital Object Identifier (DOI)

10.1038/365721a0

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