Direct observation of kinesin stepping by optical trapping interferometry.
Journal Article (Journal Article)
Do biological motors move with regular steps? To address this question, we constructed instrumentation with the spatial and temporal sensitivity to resolve movement on a molecular scale. We deposited silica beads carrying single molecules of the motor protein kinesin on microtubules using optical tweezers and analysed their motion under controlled loads by interferometry. We find that kinesin moves with 8-nm steps.
Full Text
Duke Authors
Cited Authors
- Svoboda, K; Schmidt, CF; Schnapp, BJ; Block, SM
Published Date
- October 1993
Published In
Volume / Issue
- 365 / 6448
Start / End Page
- 721 - 727
PubMed ID
- 8413650
Electronic International Standard Serial Number (EISSN)
- 1476-4687
International Standard Serial Number (ISSN)
- 0028-0836
Digital Object Identifier (DOI)
- 10.1038/365721a0
Language
- eng