Absolute quantities and equilibrium kinetics of macromolecular adsorption measured by fluorescence photobleaching in total internal reflection

Journal Article (Journal Article)

A combination of total internal reflection fluorescence and fluorescence recovery after photobleaching was used to measure the adsorption of 7-chloro-4-nitrobenz-2-oxa-1,3-diazole (NBD) labeled bovine serum albumin and NBD labeled lysozyme to plain quartz and alkylated quartz. A new method was developed to measure quantitatively the adsorbed amount of protein, using an intrinsic calibration. The results were confirmed by accompanying ellipsometry measurements. Equilibrium exchange kinetics of the adsorbed proteins between the surface and the bulk were measured as a function of temperature. © 1990.

Full Text

Duke Authors

Cited Authors

  • Zimmermann, RM; Schmidt, CF; Gaub, HE

Published Date

  • October 1, 1990

Published In

Volume / Issue

  • 139 / 1

Start / End Page

  • 268 - 280

International Standard Serial Number (ISSN)

  • 0021-9797

Digital Object Identifier (DOI)

  • 10.1016/0021-9797(90)90462-W

Citation Source

  • Scopus