Moving into the cell: single-molecule studies of molecular motors in complex environments.

Journal Article (Review;Journal Article)

Much has been learned in the past decades about molecular force generation. Single-molecule techniques, such as atomic force microscopy, single-molecule fluorescence microscopy and optical tweezers, have been key in resolving the mechanisms behind the power strokes, 'processive' steps and forces of cytoskeletal motors. However, it remains unclear how single force generators are integrated into composite mechanical machines in cells to generate complex functions such as mitosis, locomotion, intracellular transport or mechanical sensory transduction. Using dynamic single-molecule techniques to track, manipulate and probe cytoskeletal motor proteins will be crucial in providing new insights.

Full Text

Duke Authors

Cited Authors

  • Veigel, C; Schmidt, CF

Published Date

  • March 2011

Published In

Volume / Issue

  • 12 / 3

Start / End Page

  • 163 - 176

PubMed ID

  • 21326200

Electronic International Standard Serial Number (EISSN)

  • 1471-0080

International Standard Serial Number (ISSN)

  • 1471-0072

Digital Object Identifier (DOI)

  • 10.1038/nrm3062


  • eng