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Microrheology of hyaluronan solutions: implications for the endothelial glycocalyx.

Publication ,  Journal Article
Nijenhuis, N; Mizuno, D; Schmidt, CF; Vink, H; Spaan, JAE
Published in: Biomacromolecules
September 2008

The endothelial glycocalyx (EG) is a complex biopolymer network produced by vascular endothelial cells that forms a layer with multiple functions at the luminal side of blood vessels. The EG acts as an anti-adhesive protection layer, as a molecular sieve, as a chemical sensor site, and as a mechanotransducer of fluid shear stress to the underlying cell layer. A major component involved in these processes is the highly hydrated glycosaminoglycan (GAG) hyaluronan (HA). Here we used laser interferometry to measure the broadband mechanical response of reconstituted HA solutions at close to physiological conditions. HA showed rheological behavior consistent with that of a flexible polymer. The elastic behavior observed for entangled HA networks showed reptational relaxation with a large distribution of time scales, which disappeared quickly (15 min) with the addition of hyaluronidase (HAase). We conclude that the broadband mechanical probing of model systems (HA solutions) provides quantitative data that are crucial to understand the mechanical response of the EG in vivo and its role in mechanosensing.

Duke Scholars

Published In

Biomacromolecules

DOI

EISSN

1526-4602

ISSN

1525-7797

Publication Date

September 2008

Volume

9

Issue

9

Start / End Page

2390 / 2398

Related Subject Headings

  • Solutions
  • Rheology
  • Polymers
  • Molecular Weight
  • Models, Molecular
  • Hyaluronoglucosaminidase
  • Hyaluronic Acid
  • Glycocalyx
  • Enzyme Activation
  • Endothelial Cells
 

Citation

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ICMJE
MLA
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Nijenhuis, N., Mizuno, D., Schmidt, C. F., Vink, H., & Spaan, J. A. E. (2008). Microrheology of hyaluronan solutions: implications for the endothelial glycocalyx. Biomacromolecules, 9(9), 2390–2398. https://doi.org/10.1021/bm800381z
Nijenhuis, Nadja, Daisuke Mizuno, Christoph F. Schmidt, Hans Vink, and Jos A. E. Spaan. “Microrheology of hyaluronan solutions: implications for the endothelial glycocalyx.Biomacromolecules 9, no. 9 (September 2008): 2390–98. https://doi.org/10.1021/bm800381z.
Nijenhuis N, Mizuno D, Schmidt CF, Vink H, Spaan JAE. Microrheology of hyaluronan solutions: implications for the endothelial glycocalyx. Biomacromolecules. 2008 Sep;9(9):2390–8.
Nijenhuis, Nadja, et al. “Microrheology of hyaluronan solutions: implications for the endothelial glycocalyx.Biomacromolecules, vol. 9, no. 9, Sept. 2008, pp. 2390–98. Epmc, doi:10.1021/bm800381z.
Nijenhuis N, Mizuno D, Schmidt CF, Vink H, Spaan JAE. Microrheology of hyaluronan solutions: implications for the endothelial glycocalyx. Biomacromolecules. 2008 Sep;9(9):2390–2398.
Journal cover image

Published In

Biomacromolecules

DOI

EISSN

1526-4602

ISSN

1525-7797

Publication Date

September 2008

Volume

9

Issue

9

Start / End Page

2390 / 2398

Related Subject Headings

  • Solutions
  • Rheology
  • Polymers
  • Molecular Weight
  • Models, Molecular
  • Hyaluronoglucosaminidase
  • Hyaluronic Acid
  • Glycocalyx
  • Enzyme Activation
  • Endothelial Cells