Optical trap stiffness in the presence and absence of spherical aberrations.

Journal Article (Journal Article)

Optical traps are commonly constructed with high-numerical-aperture objectives. Oil-immersion objectives suffer from spherical aberrations when used for imaging in aqueous solutions. The effect of spherical aberrations on trapping strength has been modeled by approximation, and only a few experimental results are available in the case of micrometer-sized particles. We present an experimental study of the dependence of lateral and axial optical-trap stiffness on focusing depth for polystyrene and silica beads of 2 microm diameter by using oil- and water-immersion objectives. We demonstrate a strong depth dependence of trap stiffness with the oil-immersion objective, whereas no depth dependence was observed with the water-immersion objective.

Full Text

Duke Authors

Cited Authors

  • Vermeulen, KC; Wuite, GJL; Stienen, GJM; Schmidt, CF

Published Date

  • March 2006

Published In

Volume / Issue

  • 45 / 8

Start / End Page

  • 1812 - 1819

PubMed ID

  • 16572698

Pubmed Central ID

  • 16572698

Electronic International Standard Serial Number (EISSN)

  • 1539-4522

International Standard Serial Number (ISSN)

  • 1559-128X

Digital Object Identifier (DOI)

  • 10.1364/ao.45.001812


  • eng