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Viscoelastic response of a model endothelial glycocalyx.

Publication ,  Journal Article
Nijenhuis, N; Mizuno, D; Spaan, JAE; Schmidt, CF
Published in: Physical biology
July 2009

Many cells cover themselves with a multifunctional polymer coat, the pericellular matrix (PCM), to mediate mechanical interactions with the environment. A particular PCM, the endothelial glycocalyx (EG), is formed by vascular endothelial cells at their luminal side, forming a mechanical interface between the flowing blood and the endothelial cell layer. The glycosaminoglycan (GAG) hyaluronan (HA) is involved in the main functions of the EG, mechanotransduction of fluid shear stress and molecular sieving. HA, due to its length, is the only GAG in the EG or any other PCM able to form an entangled network. The mechanical functions of the EG are, however, impaired when any one of its components is removed. We here used microrheology to measure the effect of the EG constituents heparan sulfate, chondroitin sulfate, whole blood plasma and albumin on the high-bandwidth mechanical properties of a HA solution. Furthermore, we probed the effect of the hyaldherin aggrecan, a constituent of the PCM of chondrocytes, and very similar to versican (present in the PCM of various cells, and possibly in the EG). We show that components directly interacting with HA (chondroitin sulfate and aggrecan) can increase the viscoelastic shear modulus of the polymer composite.

Duke Scholars

Published In

Physical biology

DOI

EISSN

1478-3975

ISSN

1478-3967

Publication Date

July 2009

Volume

6

Issue

2

Start / End Page

025014

Related Subject Headings

  • Viscoelastic Substances
  • Shear Strength
  • Serum Albumin, Bovine
  • Rheology
  • Plasma
  • Models, Biological
  • Hyaluronic Acid
  • Heparitin Sulfate
  • Glycocalyx
  • Endothelial Cells
 

Citation

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Nijenhuis, N., Mizuno, D., Spaan, J. A. E., & Schmidt, C. F. (2009). Viscoelastic response of a model endothelial glycocalyx. Physical Biology, 6(2), 025014. https://doi.org/10.1088/1478-3975/6/2/025014
Nijenhuis, Nadja, Daisuke Mizuno, Jos A. E. Spaan, and Christoph F. Schmidt. “Viscoelastic response of a model endothelial glycocalyx.Physical Biology 6, no. 2 (July 2009): 025014. https://doi.org/10.1088/1478-3975/6/2/025014.
Nijenhuis N, Mizuno D, Spaan JAE, Schmidt CF. Viscoelastic response of a model endothelial glycocalyx. Physical biology. 2009 Jul;6(2):025014.
Nijenhuis, Nadja, et al. “Viscoelastic response of a model endothelial glycocalyx.Physical Biology, vol. 6, no. 2, July 2009, p. 025014. Epmc, doi:10.1088/1478-3975/6/2/025014.
Nijenhuis N, Mizuno D, Spaan JAE, Schmidt CF. Viscoelastic response of a model endothelial glycocalyx. Physical biology. 2009 Jul;6(2):025014.
Journal cover image

Published In

Physical biology

DOI

EISSN

1478-3975

ISSN

1478-3967

Publication Date

July 2009

Volume

6

Issue

2

Start / End Page

025014

Related Subject Headings

  • Viscoelastic Substances
  • Shear Strength
  • Serum Albumin, Bovine
  • Rheology
  • Plasma
  • Models, Biological
  • Hyaluronic Acid
  • Heparitin Sulfate
  • Glycocalyx
  • Endothelial Cells