Resolving the molecular structure of microtubules under physiological conditions with scanning force microscopy.

Journal Article (Journal Article)

We have imaged microtubules, essential structural elements of the cytoskeleton in eukaryotic cells, in physiological conditions by scanning force microscopy. We have achieved molecular resolution without the use of cross-linking and chemical fixation methods. With tip forces below 0.3 nN, protofilaments with approximately 6 nm separation could be clearly distinguished. Lattice defects in the microtubule wall were directly visible, including point defects and protofilament separations. Higher tip forces destroyed the top half of the microtubules, revealing the inner surface of the substrate-attached protofilaments. Monomers could be resolved on these inner surfaces.

Full Text

Duke Authors

Cited Authors

  • Schaap, IAT; de Pablo, PJ; Schmidt, CF

Published Date

  • August 2004

Published In

Volume / Issue

  • 33 / 5

Start / End Page

  • 462 - 467

PubMed ID

  • 14762705

Electronic International Standard Serial Number (EISSN)

  • 1432-1017

International Standard Serial Number (ISSN)

  • 0175-7571

Digital Object Identifier (DOI)

  • 10.1007/s00249-003-0386-8


  • eng