In Vitro Differentiation of CD4+ T Cell Effector and Regulatory Subsets.

Journal Article (Journal Article)

In vitro differentiation of naïve CD4+ T cells into effector and regulatory subsets offers a means to acquire large numbers of relatively homogeneous cell populations for experimentation. However, culture systems for T cell differentiation described in the literature vary widely in efficiency and complexity, limiting their comparison across studies. Here, we present a standardized and robust method for the isolation and in vitro differentiation of six CD4+ T cell subsets from mouse naïve T cells.

Full Text

Duke Authors

Cited Authors

  • Espinosa, JR; Wheaton, JD; Ciofani, M

Published Date

  • 2020

Published In

Volume / Issue

  • 2111 /

Start / End Page

  • 79 - 89

PubMed ID

  • 31933200

Electronic International Standard Serial Number (EISSN)

  • 1940-6029

Digital Object Identifier (DOI)

  • 10.1007/978-1-0716-0266-9_7


  • eng

Conference Location

  • United States