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Regulation of alpha1G T-type calcium channel gene (CACNA1G) expression during neuronal differentiation.

Publication ,  Journal Article
Bertolesi, GE; Jollimore, CAB; Shi, C; Elbaum, L; Denovan-Wright, EM; Barnes, S; Kelly, MEM
Published in: Eur J Neurosci
May 2003

Down-regulation of T-type Ca channel current and mRNA occurs following differentiation of Y79 retinoblastoma cells. To understand how the decrease in expression is linked to cell differentiation, we examined transcriptional regulation of the Cav3.1 Ca channel gene, CACNA1G. We identified two putative promoters (A and B) in 1.3 kb of cloned genomic DNA. Reverse transcriptase-polymerase chain reaction and 5' rapid amplification of cDNA ends-polymerase chain reaction analyses demonstrated that two transcripts with different 5' untranslated regions are generated by different transcription start sites, with promoter A favoured in undifferentiated cells and promoter B favoured in differentiated cells. Functional analyses of the promoter sequence revealed that both promoters are active. Enhancer and repressor sequences were identified upstream of promoter A and B, respectively. These results suggest that the down-regulation of alpha1G mRNA in differentiated Y79 cells is mediated primarily by decreased activity of promoter A, which could occur in conjunction with repression of the activity of promoter B. The decrease in T-type Ca channel expression in Y79 cells may be an essential signal affecting phenotypic maturation and expression of other ion channel subtypes in the differentiated cells.

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Published In

Eur J Neurosci

DOI

ISSN

0953-816X

Publication Date

May 2003

Volume

17

Issue

9

Start / End Page

1802 / 1810

Location

France

Related Subject Headings

  • Tumor Cells, Cultured
  • RNA, Messenger
  • Neurons
  • Neurology & Neurosurgery
  • Molecular Sequence Data
  • Humans
  • Gene Expression Regulation
  • Cell Differentiation
  • Calcium Channels, T-Type
  • Base Sequence
 

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Bertolesi, G. E., Jollimore, C. A. B., Shi, C., Elbaum, L., Denovan-Wright, E. M., Barnes, S., & Kelly, M. E. M. (2003). Regulation of alpha1G T-type calcium channel gene (CACNA1G) expression during neuronal differentiation. Eur J Neurosci, 17(9), 1802–1810. https://doi.org/10.1046/j.1460-9568.2003.02618.x
Bertolesi, Gabriel E., Christine A. B. Jollimore, Chanjuan Shi, Lindsy Elbaum, Eileen M. Denovan-Wright, Steven Barnes, and Melanie E. M. Kelly. “Regulation of alpha1G T-type calcium channel gene (CACNA1G) expression during neuronal differentiation.Eur J Neurosci 17, no. 9 (May 2003): 1802–10. https://doi.org/10.1046/j.1460-9568.2003.02618.x.
Bertolesi GE, Jollimore CAB, Shi C, Elbaum L, Denovan-Wright EM, Barnes S, et al. Regulation of alpha1G T-type calcium channel gene (CACNA1G) expression during neuronal differentiation. Eur J Neurosci. 2003 May;17(9):1802–10.
Bertolesi, Gabriel E., et al. “Regulation of alpha1G T-type calcium channel gene (CACNA1G) expression during neuronal differentiation.Eur J Neurosci, vol. 17, no. 9, May 2003, pp. 1802–10. Pubmed, doi:10.1046/j.1460-9568.2003.02618.x.
Bertolesi GE, Jollimore CAB, Shi C, Elbaum L, Denovan-Wright EM, Barnes S, Kelly MEM. Regulation of alpha1G T-type calcium channel gene (CACNA1G) expression during neuronal differentiation. Eur J Neurosci. 2003 May;17(9):1802–1810.
Journal cover image

Published In

Eur J Neurosci

DOI

ISSN

0953-816X

Publication Date

May 2003

Volume

17

Issue

9

Start / End Page

1802 / 1810

Location

France

Related Subject Headings

  • Tumor Cells, Cultured
  • RNA, Messenger
  • Neurons
  • Neurology & Neurosurgery
  • Molecular Sequence Data
  • Humans
  • Gene Expression Regulation
  • Cell Differentiation
  • Calcium Channels, T-Type
  • Base Sequence