Optimizing Nervous System-Specific Gene Targeting with Cre Driver Lines: Prevalence of Germline Recombination and Influencing Factors.

Journal Article (Journal Article)

The Cre-loxP system is invaluable for spatial and temporal control of gene knockout, knockin, and reporter expression in the mouse nervous system. However, we report varying probabilities of unexpected germline recombination in distinct Cre driver lines designed for nervous system-specific recombination. Selective maternal or paternal germline recombination is showcased with sample Cre lines. Collated data reveal germline recombination in over half of 64 commonly used Cre driver lines, in most cases with a parental sex bias related to Cre expression in sperm or oocytes. Slight differences among Cre driver lines utilizing common transcriptional control elements affect germline recombination rates. Specific target loci demonstrated differential recombination; thus, reporters are not reliable proxies for another locus of interest. Similar principles apply to other recombinase systems and other genetically targeted organisms. We hereby draw attention to the prevalence of germline recombination and provide guidelines to inform future research for the neuroscience and broader molecular genetics communities.

Full Text

Duke Authors

Cited Authors

  • Luo, L; Ambrozkiewicz, MC; Benseler, F; Chen, C; Dumontier, E; Falkner, S; Furlanis, E; Gomez, AM; Hoshina, N; Huang, W-H; Hutchison, MA; Itoh-Maruoka, Y; Lavery, LA; Li, W; Maruo, T; Motohashi, J; Pai, EL-L; Pelkey, KA; Pereira, A; Philips, T; Sinclair, JL; Stogsdill, JA; Traunmüller, L; Wang, J; Wortel, J; You, W; Abumaria, N; Beier, KT; Brose, N; Burgess, HA; Cepko, CL; Cloutier, J-F; Eroglu, C; Goebbels, S; Kaeser, PS; Kay, JN; Lu, W; Luo, L; Mandai, K; McBain, CJ; Nave, K-A; Prado, MAM; Prado, VF; Rothstein, J; Rubenstein, JLR; Saher, G; Sakimura, K; Sanes, JR; Scheiffele, P; Takai, Y; Umemori, H; Verhage, M; Yuzaki, M; Zoghbi, HY; Kawabe, H; Craig, AM

Published Date

  • April 8, 2020

Published In

Volume / Issue

  • 106 / 1

Start / End Page

  • 37 - 65.e5

PubMed ID

  • 32027825

Pubmed Central ID

  • PMC7377387

Electronic International Standard Serial Number (EISSN)

  • 1097-4199

Digital Object Identifier (DOI)

  • 10.1016/j.neuron.2020.01.008


  • eng

Conference Location

  • United States