Concentration-Dependent Recruitment of Mammalian Odorant Receptors.

Journal Article (Journal Article)

A fundamental challenge in studying principles of organization used by the olfactory system to encode odor concentration information has been identifying comprehensive sets of activated odorant receptors (ORs) across a broad concentration range inside freely behaving animals. In mammals, this has recently become feasible with high-throughput sequencing-based methods that identify populations of activated ORs in vivo In this study, we characterized the mouse OR repertoires activated by the two odorants, acetophenone (ACT) and 2,5-dihydro-2,4,5-trimethylthiazoline (TMT), from 0.01% to 100% (v/v) as starting concentrations using phosphorylated ribosomal protein S6 capture followed by RNA-Seq. We found Olfr923 to be one of the most sensitive ORs that is enriched by ACT. Using a mouse line that genetically labels Olfr923-positive axons, we provided evidence that ACT activates the Olfr923 glomeruli in the olfactory bulb. Through molecular dynamics stimulations, we identified amino acid residues in the Olfr923 binding cavity that facilitate ACT binding. This study sheds light on the active process by which unique OR repertoires may collectively facilitate the discrimination of odorant concentrations.

Full Text

Duke Authors

Cited Authors

  • Hu, XS; Ikegami, K; Vihani, A; Zhu, KW; Zapata, M; de March, CA; Do, M; Vaidya, N; Kucera, G; Bock, C; Jiang, Y; Yohda, M; Matsunami, H

Published In

Volume / Issue

  • 7 / 2

PubMed ID

  • 32015097

Pubmed Central ID

  • PMC7189481

Electronic International Standard Serial Number (EISSN)

  • 2373-2822

Digital Object Identifier (DOI)

  • 10.1523/ENEURO.0103-19.2019

Language

  • eng

Conference Location

  • United States