Skip to main content

Concentration-Dependent Recruitment of Mammalian Odorant Receptors.

Publication ,  Journal Article
Hu, XS; Ikegami, K; Vihani, A; Zhu, KW; Zapata, M; de March, CA; Do, M; Vaidya, N; Kucera, G; Bock, C; Jiang, Y; Yohda, M; Matsunami, H
Published in: eNeuro
2020

A fundamental challenge in studying principles of organization used by the olfactory system to encode odor concentration information has been identifying comprehensive sets of activated odorant receptors (ORs) across a broad concentration range inside freely behaving animals. In mammals, this has recently become feasible with high-throughput sequencing-based methods that identify populations of activated ORs in vivo In this study, we characterized the mouse OR repertoires activated by the two odorants, acetophenone (ACT) and 2,5-dihydro-2,4,5-trimethylthiazoline (TMT), from 0.01% to 100% (v/v) as starting concentrations using phosphorylated ribosomal protein S6 capture followed by RNA-Seq. We found Olfr923 to be one of the most sensitive ORs that is enriched by ACT. Using a mouse line that genetically labels Olfr923-positive axons, we provided evidence that ACT activates the Olfr923 glomeruli in the olfactory bulb. Through molecular dynamics stimulations, we identified amino acid residues in the Olfr923 binding cavity that facilitate ACT binding. This study sheds light on the active process by which unique OR repertoires may collectively facilitate the discrimination of odorant concentrations.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

eNeuro

DOI

EISSN

2373-2822

Publication Date

2020

Volume

7

Issue

2

Location

United States

Related Subject Headings

  • Smell
  • Receptors, Odorant
  • Olfactory Receptor Neurons
  • Olfactory Bulb
  • Odorants
  • Mammals
  • Animals
  • 1109 Neurosciences
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Hu, X. S., Ikegami, K., Vihani, A., Zhu, K. W., Zapata, M., de March, C. A., … Matsunami, H. (2020). Concentration-Dependent Recruitment of Mammalian Odorant Receptors. ENeuro, 7(2). https://doi.org/10.1523/ENEURO.0103-19.2019
Hu, Xiaoyang Serene, Kentaro Ikegami, Aashutosh Vihani, Kevin W. Zhu, Marcelo Zapata, Claire A. de March, Matthew Do, et al. “Concentration-Dependent Recruitment of Mammalian Odorant Receptors.ENeuro 7, no. 2 (2020). https://doi.org/10.1523/ENEURO.0103-19.2019.
Hu XS, Ikegami K, Vihani A, Zhu KW, Zapata M, de March CA, et al. Concentration-Dependent Recruitment of Mammalian Odorant Receptors. eNeuro. 2020;7(2).
Hu, Xiaoyang Serene, et al. “Concentration-Dependent Recruitment of Mammalian Odorant Receptors.ENeuro, vol. 7, no. 2, 2020. Pubmed, doi:10.1523/ENEURO.0103-19.2019.
Hu XS, Ikegami K, Vihani A, Zhu KW, Zapata M, de March CA, Do M, Vaidya N, Kucera G, Bock C, Jiang Y, Yohda M, Matsunami H. Concentration-Dependent Recruitment of Mammalian Odorant Receptors. eNeuro. 2020;7(2).

Published In

eNeuro

DOI

EISSN

2373-2822

Publication Date

2020

Volume

7

Issue

2

Location

United States

Related Subject Headings

  • Smell
  • Receptors, Odorant
  • Olfactory Receptor Neurons
  • Olfactory Bulb
  • Odorants
  • Mammals
  • Animals
  • 1109 Neurosciences