Scope and Predictive Genetic/Phenotypic Signatures of Bicarbonate (NaHCO3) Responsiveness and β-Lactam Sensitization in Methicillin-Resistant Staphylococcus aureus.
Journal Article (Journal Article)
Addition of sodium bicarbonate (NaHCO3) to standard antimicrobial susceptibility testing medium reveals certain methicillin-resistant Staphylococcus aureus (MRSA) strains to be highly susceptible to β-lactams. We investigated the prevalence of this phenotype (NaHCO3 responsiveness) to two β-lactams among 58 clinical MRSA bloodstream isolates. Of note, ∼75% and ∼36% of isolates displayed the NaHCO3 responsiveness phenotype to cefazolin (CFZ) and oxacillin (OXA), respectively. Neither intrinsic β-lactam MICs in standard Mueller-Hinton broth (MHB) nor population analysis profiles were predictive of this phenotype. Several genotypic markers (clonal complex 8 [CC8]; agr I and spa t008) were associated with NaHCO3 responsiveness for OXA.
Full Text
Duke Authors
Cited Authors
- Ersoy, SC; Otmishi, M; Milan, VT; Li, L; Pak, Y; Mediavilla, J; Chen, L; Kreiswirth, B; Chambers, HF; Proctor, RA; Xiong, YQ; Fowler, VG; Bayer, AS
Published Date
- April 21, 2020
Published In
Volume / Issue
- 64 / 5
PubMed ID
- 32041719
Pubmed Central ID
- PMC7179597
Electronic International Standard Serial Number (EISSN)
- 1098-6596
Digital Object Identifier (DOI)
- 10.1128/AAC.02445-19
Language
- eng
Conference Location
- United States