Hydrogen peroxide sensor HPCA1 is an LRR receptor kinase in Arabidopsis.

Journal Article (Journal Article)

Hydrogen peroxide (H2 O2 ) is a major reactive oxygen species in unicellular and multicellular organisms, and is produced extracellularly in response to external stresses and internal cues1-4 . H2 O2 enters cells through aquaporin membrane proteins and covalently modifies cytoplasmic proteins to regulate signalling and cellular processes. However, whether sensors for H2 O2 also exist on the cell surface remains unknown. In plant cells, H2 O2 triggers an influx of Ca2+ ions, which is thought to be involved in H2 O2 sensing and signalling. Here, by using forward genetic screens based on Ca2+ imaging, we isolated hydrogen-peroxide-induced Ca2+ increases (hpca) mutants in Arabidopsis, and identified HPCA1 as a leucine-rich-repeat receptor kinase belonging to a previously uncharacterized subfamily that features two extra pairs of cysteine residues in the extracellular domain. HPCA1 is localized to the plasma membrane and is activated by H2 O2 via covalent modification of extracellular cysteine residues, which leads to autophosphorylation of HPCA1. HPCA1 mediates H2 O2 -induced activation of Ca2+ channels in guard cells and is required for stomatal closure. Our findings help to identify how the perception of extracellular H2 O2 is integrated with responses to various external stresses and internal cues in plants, and have implications for the design of crops with enhanced fitness.

Full Text

Duke Authors

Cited Authors

  • Wu, F; Chi, Y; Jiang, Z; Xu, Y; Xie, L; Huang, F; Wan, D; Ni, J; Yuan, F; Wu, X; Zhang, Y; Wang, L; Ye, R; Byeon, B; Wang, W; Zhang, S; Sima, M; Chen, S; Zhu, M; Pei, J; Johnson, DM; Zhu, S; Cao, X; Pei, C; Zai, Z; Liu, Y; Liu, T; Swift, GB; Zhang, W; Yu, M; Hu, Z; Siedow, JN; Chen, X; Pei, Z-M

Published Date

  • February 19, 2020

Published In

Volume / Issue

  • 578 / 7796

Start / End Page

  • 577 - 581

PubMed ID

  • 32076270

Pubmed Central ID

  • 32076270

Electronic International Standard Serial Number (EISSN)

  • 1476-4687

International Standard Serial Number (ISSN)

  • 0028-0836

Digital Object Identifier (DOI)

  • 10.1038/s41586-020-2032-3

Language

  • eng