Application of 15 N2 -Diazirines as a Versatile Platform for Hyperpolarization of Biological Molecules by d-DNP.

Journal Article (Journal Article)

15 N2 -Diazirines represent an attractive class of imaging tags for hyperpolarized magnetic resonance imaging (HP-MRI), offering desirable biocompatibility, ease of incorporation into a variety of molecules, and ability to deliver long-lasting polarization. We have recently established hyperpolarization of 15 N2 -diazirines in organic solvents using SABRE-Shield Enables Alignment Transfer to Heteronuclei (SABRE-SHEATH). Yet, the current challenge of SABRE-SHEATH in water, specifically poor polarization efficiency, presents a barrier in examining the practical use of 15 N2 -diazirines for HP-MRI. Herein, we show that efficient polarization of diverse 15 N2 -diazirine-labeled molecules in water can be readily achieved by dissolution dynamic nuclear polarization (d-DNP), a hyperpolarization technique used in clinical practice. Hyperpolarization by d-DNP also demonstrates greater enhancement for long-lasting 15 N signals, in comparison with SABRE-SHEATH. Various biologically important molecules are studied in this work, including amino acid, sugar, and drug compounds, demonstrating the great potential of 15 N2 -diazirines as molecular tags in broad biomedical and clinical applications.

Full Text

Duke Authors

Cited Authors

  • Park, H; Zhang, G; Bae, J; Theis, T; Warren, WS; Wang, Q

Published Date

  • March 2020

Published In

Volume / Issue

  • 31 / 3

Start / End Page

  • 537 - 541

PubMed ID

  • 32023034

Pubmed Central ID

  • PMC7409511

Electronic International Standard Serial Number (EISSN)

  • 1520-4812

International Standard Serial Number (ISSN)

  • 1043-1802

Digital Object Identifier (DOI)

  • 10.1021/acs.bioconjchem.0c00028

Language

  • eng