Genetic Analysis Reveals AMPK Is Required to Support Tumor Growth in Murine Kras-Dependent Lung Cancer Models.

Journal Article

AMPK, a conserved sensor of low cellular energy, can either repress or promote tumor growth depending on the context. However, no studies have examined AMPK function in autochthonous genetic mouse models of epithelial cancer. Here, we examine the role of AMPK in murine KrasG12D -mediated non-small-cell lung cancer (NSCLC), a cancer type in humans that harbors frequent inactivating mutations in the LKB1 tumor suppressor-the predominant upstream activating kinase of AMPK and 12 related kinases. Unlike LKB1 deletion, AMPK deletion in KrasG12D lung tumors did not accelerate lung tumor growth. Moreover, deletion of AMPK in KrasG12D p53f/f tumors reduced lung tumor burden. We identified a critical role for AMPK in regulating lysosomal gene expression through the Tfe3 transcription factor, which was required to support NSCLC growth. Thus, AMPK supports the growth of KrasG12D -dependent lung cancer through the induction of lysosomes, highlighting an unrecognized liability of NSCLC.

Full Text

Duke Authors

Cited Authors

  • Eichner, LJ; Brun, SN; Herzig, S; Young, NP; Curtis, SD; Shackelford, DB; Shokhirev, MN; Leblanc, M; Vera, LI; Hutchins, A; Ross, DS; Shaw, RJ; Svensson, RU

Published Date

  • February 2019

Published In

Volume / Issue

  • 29 / 2

Start / End Page

  • 285 - 302.e7

PubMed ID

  • 30415923

Pubmed Central ID

  • 30415923

Electronic International Standard Serial Number (EISSN)

  • 1932-7420

International Standard Serial Number (ISSN)

  • 1550-4131

Digital Object Identifier (DOI)

  • 10.1016/j.cmet.2018.10.005

Language

  • eng