The Bcl6-SMRT/NCoR cistrome represses inflammation to attenuate atherosclerosis.

Published

Journal Article

Chronic inflammation is a hallmark of atherosclerosis, but its transcriptional underpinnings are poorly understood. We show that the transcriptional repressor Bcl6 is an anti-inflammatory regulator whose loss in bone marrow of Ldlr(-/-) mice results in severe atherosclerosis and xanthomatous tendonitis, a virtually pathognomonic complication in patients with familial hypercholesterolemia. Disruption of the interaction between Bcl6 and SMRT or NCoR with a peptide inhibitor in vitro recapitulated atherogenic gene changes in mice transplanted with Bcl6-deficient bone marrow, pointing to these cofactors as key mediators of Bcl6 inflammatory suppression. Using ChIP-seq, we reveal the SMRT and NCoR corepressor cistromes, each consisting of over 30,000 binding sites with a nearly 50% overlap. While the complete cistromes identify a diversity of signaling pathways, the Bcl6-bound subcistromes for each corepressor are highly enriched for NF-κB-driven inflammatory and tissue remodeling genes. These results reveal that Bcl6-SMRT/NCoR complexes constrain immune responses and contribute to the prevention of atherosclerosis.

Full Text

Duke Authors

Cited Authors

  • Barish, GD; Yu, RT; Karunasiri, MS; Becerra, D; Kim, J; Tseng, TW; Tai, L-J; Leblanc, M; Diehl, C; Cerchietti, L; Miller, YI; Witztum, JL; Melnick, AM; Dent, AL; Tangirala, RK; Evans, RM

Published Date

  • April 2012

Published In

Volume / Issue

  • 15 / 4

Start / End Page

  • 554 - 562

PubMed ID

  • 22465074

Pubmed Central ID

  • 22465074

Electronic International Standard Serial Number (EISSN)

  • 1932-7420

International Standard Serial Number (ISSN)

  • 1550-4131

Digital Object Identifier (DOI)

  • 10.1016/j.cmet.2012.02.012

Language

  • eng