Time-restricted feeding without reducing caloric intake prevents metabolic diseases in mice fed a high-fat diet.


Journal Article

While diet-induced obesity has been exclusively attributed to increased caloric intake from fat, animals fed a high-fat diet (HFD) ad libitum (ad lib) eat frequently throughout day and night, disrupting the normal feeding cycle. To test whether obesity and metabolic diseases result from HFD or disruption of metabolic cycles, we subjected mice to either ad lib or time-restricted feeding (tRF) of a HFD for 8 hr per day. Mice under tRF consume equivalent calories from HFD as those with ad lib access yet are protected against obesity, hyperinsulinemia, hepatic steatosis, and inflammation and have improved motor coordination. The tRF regimen improved CREB, mTOR, and AMPK pathway function and oscillations of the circadian clock and their target genes' expression. These changes in catabolic and anabolic pathways altered liver metabolome and improved nutrient utilization and energy expenditure. We demonstrate in mice that tRF regimen is a nonpharmacological strategy against obesity and associated diseases.

Full Text

Duke Authors

Cited Authors

  • Hatori, M; Vollmers, C; Zarrinpar, A; DiTacchio, L; Bushong, EA; Gill, S; Leblanc, M; Chaix, A; Joens, M; Fitzpatrick, JAJ; Ellisman, MH; Panda, S

Published Date

  • June 2012

Published In

Volume / Issue

  • 15 / 6

Start / End Page

  • 848 - 860

PubMed ID

  • 22608008

Pubmed Central ID

  • 22608008

Electronic International Standard Serial Number (EISSN)

  • 1932-7420

International Standard Serial Number (ISSN)

  • 1550-4131

Digital Object Identifier (DOI)

  • 10.1016/j.cmet.2012.04.019


  • eng