The Impact of Sustained Immunization Regimens on the Antibody Response to Oligomannose Glycans.

Journal Article (Journal Article)

The high mannose patch (HMP) of the HIV envelope protein (Env) is the structure most frequently targeted by broadly neutralizing antibodies; therefore, many researchers have attempted to use mimics of this region as a vaccine immunogen. In our previous efforts, vaccinating rabbits with evolved HMP mimic glycopeptides containing Man9 resulted in an overall antibody response targeting the glycan core and linker rather than the full glycan or Manα1→2Man tips of Man9 glycans. A possible reason could be processing of our immunogen by host serum mannosidases. We sought to test whether more prolonged dosing could increase the antibody response to intact glycans, possibly by increasing the availability of intact Man9 to germinal centers. Here, we describe a study investigating the impact of immunization regimen on antibody response by testing immunogen delivery through bolus, an exponential series of mini doses, or a continuously infusing mini-osmotic pump. Our results indicate that, with our glycopeptide immunogens, standard bolus immunization elicited the strongest HIV Env-binding antibody response, even though higher overall titers to the glycopeptide were elicited by the exponential and pump regimens. Antibody selectivity for intact glycan was, if anything, slightly better in the bolus-immunized animals.

Full Text

Duke Authors

Cited Authors

  • Nguyen, DN; Redman, RL; Horiya, S; Bailey, JK; Xu, B; Stanfield, RL; Temme, JS; LaBranche, CC; Wang, S; Rodal, AA; Montefiori, DC; Wilson, IA; Krauss, IJ

Published Date

  • March 20, 2020

Published In

Volume / Issue

  • 15 / 3

Start / End Page

  • 789 - 798

PubMed ID

  • 32109354

Pubmed Central ID

  • PMC7091532

Electronic International Standard Serial Number (EISSN)

  • 1554-8937

Digital Object Identifier (DOI)

  • 10.1021/acschembio.0c00053


  • eng

Conference Location

  • United States