Interleukin-6: effects on and production by rat granulosa cells in vitro.

Journal Article (Journal Article)

Treatment of FSH-stimulated granulosa cells with increasing amounts of interleukin-6 (IL-6) caused a significant concentration-dependent suppression of progesterone biosynthesis. However, basal progesterone production in non-FSH-stimulated cells remained unresponsive to the cytokine. Quantitation of IL-6 in the conditioned media from untreated granulosa cells by the 7TD1 hybridoma cell bioassay revealed detectable levels of IL-6. Further, FSH treatment caused a significant concentration-dependent increase in IL-6 release. In contrast, both basal and FSH-stimulated IL-6 release could be significantly suppressed by interferon gamma (INF-gamma). The results of the present study suggest: 1) a role for IL-6 in the regulation of progesterone production, 2) that the granulosa cell is a source of IL-6 and 3) that the release of IL-6 by the granulosa cell is a regulated event.

Full Text

Duke Authors

Cited Authors

  • Gorospe, WC; Hughes, FM; Spangelo, BL

Published Date

  • March 1992

Published In

Volume / Issue

  • 130 / 3

Start / End Page

  • 1750 - 1752

PubMed ID

  • 1537322

International Standard Serial Number (ISSN)

  • 0013-7227

Digital Object Identifier (DOI)

  • 10.1210/endo.130.3.1537322


  • eng

Conference Location

  • United States