Interleukin-6: effects on and production by rat granulosa cells in vitro.
Journal Article (Journal Article)
Treatment of FSH-stimulated granulosa cells with increasing amounts of interleukin-6 (IL-6) caused a significant concentration-dependent suppression of progesterone biosynthesis. However, basal progesterone production in non-FSH-stimulated cells remained unresponsive to the cytokine. Quantitation of IL-6 in the conditioned media from untreated granulosa cells by the 7TD1 hybridoma cell bioassay revealed detectable levels of IL-6. Further, FSH treatment caused a significant concentration-dependent increase in IL-6 release. In contrast, both basal and FSH-stimulated IL-6 release could be significantly suppressed by interferon gamma (INF-gamma). The results of the present study suggest: 1) a role for IL-6 in the regulation of progesterone production, 2) that the granulosa cell is a source of IL-6 and 3) that the release of IL-6 by the granulosa cell is a regulated event.
Full Text
Duke Authors
Cited Authors
- Gorospe, WC; Hughes, FM; Spangelo, BL
Published Date
- March 1992
Published In
Volume / Issue
- 130 / 3
Start / End Page
- 1750 - 1752
PubMed ID
- 1537322
International Standard Serial Number (ISSN)
- 0013-7227
Digital Object Identifier (DOI)
- 10.1210/endo.130.3.1537322
Language
- eng
Conference Location
- United States