Modulation of cerebral microvascular permeability by endothelial nicotinic acetylcholine receptors.
Journal Article (Journal Article)
Nicotine increases the permeability of the blood-brain barrier in vivo. This implies a possible role for nicotinic acetylcholine receptors in the regulation of cerebral microvascular permeability. Expression of nicotinic acetylcholine receptor subunits in cerebral microvessels was investigated with immunofluorescence microscopy. Positive immunoreactivity was found for receptor subunits alpha3, alpha5, alpha7, and beta2, but not subunits alpha4, beta3, or beta4. Blood-brain barrier permeability was assessed via in situ brain perfusion with [14C]sucrose. Nicotine increased the rate of sucrose entry into the brain from 0.3 +/- 0.1 to 1.1 +/- 0.2 microl.g(-1).min(-1), as previously described. This nicotine-induced increase in blood-brain barrier permeability was significantly attenuated by both the blood-brain barrier-permeant nicotinic antagonist mecamylamine and the blood-brain barrier-impermeant nicotinic antagonist hexamethonium to 0.5 +/- 0.2 and 0.3 +/- 0.2 microl.g(-1).min(-1), respectively. These data suggest that nicotinic acetylcholine receptors expressed on the cerebral microvascular endothelium mediate nicotine-induced changes in blood-brain barrier permeability.
Full Text
Duke Authors
Cited Authors
- Hawkins, BT; Egleton, RD; Davis, TP
Published Date
- July 2005
Published In
Volume / Issue
- 289 / 1
Start / End Page
- H212 - H219
PubMed ID
- 15708958
Electronic International Standard Serial Number (EISSN)
- 1522-1539
International Standard Serial Number (ISSN)
- 0363-6135
Digital Object Identifier (DOI)
- 10.1152/ajpheart.01210.2004
Language
- eng