Decreased blood-brain barrier permeability to fluorescein in streptozotocin-treated rats.

Journal Article (Journal Article)

Investigations of the blood-brain barrier (BBB) in diabetes have yielded contradictory results. It is possible that diabetes differentially affects paracellular and transcellular permeabilities via modulation of tight junction and transport proteins, respectively. Fluorescein (FL), a marker for paracellular permeability, is a substrate for the transport proteins organic anion transporter (OAT)-3 and multidrug resistance protein (MRP)-2 at the BBB. Furthermore, MRP-2-mediated efflux of FL can be upregulated by glucose. In this study, streptozotocin-induced diabetes led to decreased brain distribution of FL measured by in situ brain perfusion, consistent with activation of an efflux transport system for FL at the BBB. This change was paralleled by increased protein expression of MRP-2, but not OAT-3, in cerebral microvessels. These data indicate that diabetes may lead to changes in efflux transporters at the BBB and have implications for delivery of therapeutics to the central nervous system.

Full Text

Duke Authors

Cited Authors

  • Hawkins, BT; Ocheltree, SM; Norwood, KM; Egleton, RD

Published Date

  • January 1, 2007

Published In

Volume / Issue

  • 411 / 1

Start / End Page

  • 1 - 5

PubMed ID

  • 17110033

Pubmed Central ID

  • PMC1785293

Electronic International Standard Serial Number (EISSN)

  • 1872-7972

International Standard Serial Number (ISSN)

  • 0304-3940

Digital Object Identifier (DOI)

  • 10.1016/j.neulet.2006.09.010


  • eng