Application of genetically modified animals in bone research

Published

Book Section

© 2020 Elsevier Inc. All rights reserved. Genetically modified mice have had an enormous impact on our understanding of skeletal development, maintenance, and disease. Publicly supported efforts to generate and phenotype genes in the mouse have greatly expanded the ease of identifying and characterizing genes that play a role in skeletal tissues. CRISPR/Cas9 technology has led to rapid and cost-effective generation of mice carrying specific mutations. Conditional approaches, which enable cell-specific or temporal control over gene ablation, are the most powerful and precise means of genetic analysis. However, these approaches rely on the use of tissue-selective promoters. Understanding the temporal and spatial dynamics of these extensively used promoters is therefore vital to interpreting phenotypes or lineage studies in mice. We discuss some general features of transgenic overexpression and loss-of-function approaches, large-scale mutagenesis efforts and resources, and some of the most commonly used tissue-selective promoters and transgenic strains for tissue-specific gene analysis in skeletal cells.

Full Text

Duke Authors

Cited Authors

  • Hilton, MJ; Lyons, KM

Published Date

  • January 1, 2019

Book Title

  • Principles of Bone Biology

Start / End Page

  • 1787 - 1800

International Standard Book Number 13 (ISBN-13)

  • 9780128148419

Digital Object Identifier (DOI)

  • 10.1016/B978-0-12-814841-9.00077-4

Citation Source

  • Scopus