Mast cell stabilization improves survival by preventing apoptosis in sepsis.

Published

Journal Article

Inhibiting single cytokines produced modest effects in clinical trials, in part because the cytokines were not specific for sepsis, and sepsis may require cellular strategies. Previous studies reported that mast cells (MCs) fight infections in early sepsis. In this study, we report that MC stabilizers restrain serum TNF levels and improve survival in wild-type but not in MC-deficient mice. Yet, MC depletion in knockout mice attenuates serum TNF but does not improve survival in sepsis. Serum HMGB1 was the only factor correlating with survival. MC stabilizers inhibit systemic HMGB1 levels and rescue mice from established peritonitis. MC stabilizers fail to inhibit HMGB1 secretion from macrophages, but they prevent apoptosis and caspase-3 activation in sepsis. These results suggest that MC stabilization provides therapeutic benefits in sepsis by inhibiting extracellular release of HMGB1 from apoptotic cells. Our study provides the first evidence that MCs have major immunological implications regulating cell death in sepsis and represent a pharmacological target for infectious disorders in a clinically realistic time frame.

Full Text

Duke Authors

Cited Authors

  • Ramos, L; Peña, G; Cai, B; Deitch, EA; Ulloa, L

Published Date

  • July 1, 2010

Published In

Volume / Issue

  • 185 / 1

Start / End Page

  • 709 - 716

PubMed ID

  • 20519642

Pubmed Central ID

  • 20519642

Electronic International Standard Serial Number (EISSN)

  • 1550-6606

Digital Object Identifier (DOI)

  • 10.4049/jimmunol.1000273

Language

  • eng

Conference Location

  • United States