Regulation of murine arthritis by systemic, spinal, and intra-articular adrenoceptors.

Published

Journal Article

BACKGROUND: The regulation of the immune system by the sympathetic nervous system is allowing the design of novel treatments for inflammatory disorders such as arthritis. In this study, we have analyzed the effects of α- and β-adrenoceptor agonists injected subcutaneously, intrathecally, or intra-articularly in zymosan-induced arthritis. METHODS: Murine arthritis was induced by intra-articular (knee joint) injection of zymosan. α1 (phenylephrine), α2 (clonidine), β1 (dobutamine), or β2 (salbutamol)-adrenoceptor agonists were injected subcutaneously (sc), intrathecally (it), or intra-articularly (ia) to activate peripheral, spinal, or intra-articular adrenoceptors and to study their effects on articular edema formation and neutrophil migration into the synovial cavity. RESULTS: Treatments with phenylephrine did not affect the edema formation, but it increased neutrophil migration when injected subcutaneously (155.3%) or intra-articularly (187.7%). Treatments with clonidine inhibited neutrophil migration (59.9% sc, 68.7% it, 42.8% ia) regardless of the route of administration, but it inhibited edema formation only when injected intrathecally (66.7%) or intra-articularly (36%) but not subcutaneously. Treatments with dobutamine inhibited both edema (42.0% sc, 69.5% it, 61.6% ia) and neutrophil migration (28.4% sc, 70.3% it, 82.4% ia) in a concentration dependent manner. Likewise, all the treatments with salbutamol also inhibited edema formation (89.9% sc, 62.4% it, 69.8% ia) and neutrophil migration (76.6% sc, 39.1% it, 71.7% ia). CONCLUSION: Whereas the β-adrenoceptor agonists induced anti-inflammatory effects regardless of their route of administration, α1- and α2-adrenoceptor agonists induced either pro- and anti-inflammatory effects, respectively.

Full Text

Duke Authors

Cited Authors

  • Kanashiro, A; Leoncio, TODL; Schneider, AH; Alves, HR; Bassi, GS; Dutra, SGV; Cunha, FDQ; Ulloa, L; Malvar, DDC

Published Date

  • December 2019

Published In

Volume / Issue

  • 71 / 6

Start / End Page

  • 1095 - 1103

PubMed ID

  • 31629939

Pubmed Central ID

  • 31629939

International Standard Serial Number (ISSN)

  • 1734-1140

Digital Object Identifier (DOI)

  • 10.1016/j.pharep.2019.06.010

Language

  • eng

Conference Location

  • Switzerland